Abstract
A comparison was made of patterns of 4-hydroxycinnamic acid: CoA ligase (CoA ligase, E.C.6.2.1.12) activities in near isogenic maize cultivars with differential resistance (B73 Ht rkm ) and susceptibility (B73 Ht) to Helminthosporium maydis. CoA ligase activity was assayed with p-coumaric, caffeic and ferulic acids as substrates in fractions collected after molecular sieve column chromatography of protein extracts from maize mesocotyls. All CoA ligase activity eluted between a molecular size range of 42–77 kilodaltons. The CoA ligase elution profiles distinguished multiple peaks of activity and were different in uninoculated susceptible and resistant cultivars. Inoculation with H. maydis changed the elution profiles of the enzyme activities from the respective cultivars. The multiple peaks of enzyme activity in the elution profiles and their shift in response to infection indicate CoA ligase isoenzyme responses in these host-parasite interactions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
