Abstract
A method was developed for high-speed liquid chromatographic determination of asparagusate in asparagus shoot. The in vivo concentration of asparagusate and the activities of asparagusate dehydrogenase and lipoyl dehydrogenase (EC 1.6.4.3) in etiolated and green shoots of asparagus (Asparagus officinalis L.) were measured. The region around the apices of the shoots had higher levels of asparagusate, asparagusate dehydrogenase, and lipoyl dehydrogenase than other regions. The level of asparagusate in the etiolated shoots was lower than that in the green shoots, whereas the levels of asparagusate dehydrogenase and lipoyl dehydrogenase in the etiolated shoots were higher than those in the green shoots. Asparagusate was localized in the soluble fraction and asparagusate dehydrogenase and lipoyl dehydrogenase activities were localized in the mitochondrial fraction. Furthermore, asparagusate was found in all the various asparagus species tested.
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