Abstract

BackgroundIdentification of specific biomarkers is important for the diagnosis and treatment of non-small cell lung cancer (NSCLC). HOXC6 is a homeodomain-containing transcription factor that is highly expressed in several human cancers; however, its role in NSCLC remains unknown.MethodsThe expression and protein levels of HOXC6 were assessed in NSCLC tissue samples by Quantitative real-time PCR (qRT-PCR) and immunohistochemistry, respectively. HOXC6 was transfected into the NSCLC cell lines A549 and PC9, and used to investigate its effect on proliferation, migration, and invasion using CFSE, wound healing, and Matrigel invasion assays. Next-generation sequencing was also used to identify downstream targets of HOXC6 and to gain insights into the molecular mechanisms underlying its biological function.ResultsHOXC6 expression was significantly increased in 66.6% (20/30) of NSCLC tumor samples in comparison to normal controls. HOXC6 promoted proliferation, migration, and invasion of NSCLC cells in vitro. RNA-seq analysis demonstrated the upregulation of 310 and 112 genes in A549-HOXC6 and PC9-HOXC6 cells, respectively, and the downregulation of 665 and 385 genes in A549-HOXC6 and PC9-HOXC6 cells, respectively. HOXC6 was also found to regulate the expression of genes such as CEACAM6, SPARC, WNT6, CST1, MMP2, and KRT13, which have documented pro-tumorigenic functions.DiscussionHOXC6 is highly expressed in NSCLC, and it may enhance lung cancer progression by regulating the expression of pro-tumorigenic genes involved in proliferation, migration, and invasion. Our study highlighted the oncogenic potential of HOXC6, and suggests that it may be a novel biomarker for the diagnosis and treatment of NSCLC.

Highlights

  • IntroductionNon-small cell lung cancer (NSCLC) accounts for 80–85% of the total number of lung cancer cases (Lee et al, 2013; Zienolddiny & Skaug, 2011)

  • Lung cancer has the highest cancer incidence in the world (Chen et al, 2016b)

  • IHC analysis was consistent with the gene expression data, as HOXC6 protein was elevated in non-small cell lung cancer (NSCLC) tumor samples in comparison to adjacent normal controls (Figs. 1D and 1E)

Read more

Summary

Introduction

Non-small cell lung cancer (NSCLC) accounts for 80–85% of the total number of lung cancer cases (Lee et al, 2013; Zienolddiny & Skaug, 2011). Evidence for an oncogenic role of HOXC6 in human non-small cell lung cancer. More effective molecular makers are needed for the diagnosis and treatment of NSCLC. Identification of specific biomarkers is important for the diagnosis and treatment of non-small cell lung cancer (NSCLC). HOXC6 is a homeodomaincontaining transcription factor that is highly expressed in several human cancers; its role in NSCLC remains unknown. HOXC6 promoted proliferation, migration, and invasion of NSCLC cells in vitro. HOXC6 is highly expressed in NSCLC, and it may enhance lung cancer progression by regulating the expression of pro-tumorigenic genes involved in proliferation, migration, and invasion. Our study highlighted the oncogenic potential of HOXC6, and suggests that it may be a novel biomarker for the diagnosis and treatment of NSCLC

Methods
Results
Discussion
Conclusion
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.