Abstract
The transport of phenylalanine by the general aromatic amino acid system and of threonine by a separate system in spheroplasts of Escherichia coli 9723 is stimulated by tRNA ( E. coli B) and inhibited competitively by the respective aminoacylated tRNA. tRNA appears to increase the affinity of the transport system for the amino acid. The stimulatory activity of tRNA was found to reside in fractions containing minor isoacceptor species obtained by fractionation of the respective aminoacylated tRNA and deacylation.
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