EVALUATION OF THE YIELDS AND ANTIOXIDANT ACTIVITIES OF ESSENTIAL OILS, FIXED OILS AND EXTRACTS OBTAINED USING CONVENTIONAL METHODS FROM NIGELLA SATIVA L. AND NIGELLA DAMASCENA L. SEEDS

Background:ArtemisiadesertiKrasch belongs toAsteraceaefamily, and has many medicinal properties that used to treat a variety of diseases, including antihypertensive, invigorating blood circulation, antiallergy, antiviral, antitumor, and antioxidant.Methods:The objective of this study was the evaluation of cytotoxic and antioxidant activities as well as the chemical composition ofA.desertiessential oil extracted by different extraction methods. These extraction methods included hydro-distillation (HD), salt-HD (Salt pretreatment followed by HD), maceration-HD (maceration pretreatment followed by HD), acid-HD (acid pretreatment followed by HD), ultrasound-HD (ultrasound pretreatment followed by HD), and enzyme-HD (enzyme pretreatment followed by HD).Results:The results revealed that the highest yield of essential oil was achieved by acid-HD (0.48% ± 0.11%). In all methods, the main compounds of essential oil were camphor (44.32%–66.80%), piperiton (14.11%–24.33%), and 1,8-cineole (4.85%–6.75%). The antioxidant property of essential oils was investigated using the DPPH method. Based on the results, the essential oils extracted by acid-HD and enzyme-HD methods had the highest property. The cytotoxic property ofA.desertiessential oils was evaluated. The results demonstrated that the essential oil extracted by enzyme-HD indicated the highest cytotoxicity activity against human cervical carcinoma (HeLa) cells in both 24 and 48 h. Based on the gas chromatography-mass spectrometry results, 1,8-cineol, carvacrol, thymol, and myristicin were rich in the essential oil extracted by enzyme-HD, and the highest cytotoxicity activity of essential oil obtained by enzyme-HD method is probably related to these compounds.Conclusion:In addition, it was observed that enzyme-HD is an effective method in the extraction of essential oil with the highest antioxidant and cytotoxic activities.

Phaleria macrocarpa (Scheff.) Boerl., locally known as ‘Mahkota Dewa’, is a fruit commonly found in Malaysia. It demonstrates high potential for various biological activities, including antioxidant, anti-inflammatory, antibacterial, and anticancer properties. Nevertheless, there is a lack of research on the effect of essential oil extraction using different solvents on the antibacterial and antioxidant activities of this seed's fruit. This study aims to investigate the extraction yield, volatile components, antibacterial, and antioxidant activities of essential oil extracted from P. macrocarpa seeds using ethyl acetate, hexane, and petroleum ether as solvents. Gas chromatography-mass spectrometry was used to analyse the volatile components, while the antibacterial activity was assessed against Listeria innocua and Serratia marcescens. The antioxidant activity was evaluated using total phenolic content (TPC), total flavonoid content (TFC), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Hexane showed the highest extraction yield (51.87 ± 0.21%), followed by petroleum ether (44.40 ± 0.14%) and ethyl acetate (27.08 ± 0.10%). The essential oils extracted with ethyl acetate, hexane, and petroleum ether contained 24, 26, and 21 volatile components, respectively. The major component, 1-(ethenylthio)-octane was found in concentrations ranging from 42.371 to 54.863 ppm, followed by 2,2-dimethyl-5-(3-methyl-2-oxiranyl)cyclohexanone, with concentrations between 30.771 and 40.783 ppm. The essential oil exhibited moderate antibacterial activity, as indicated by the zone of inhibition against L. innocua (8.00 ± 1.30 mm to 11.00 ± 1.40 mm) and S. marcescens (9.00 ± 1.00 mm to 14.00 ± 1.20 mm) compared to the positive control. Additionally, the essential oil demonstrated good antioxidant activity, with TPC, TFC, and DPPH values for hexane (65.56 ± 0.02 mg GAE/g, 4.46 ± 0.17 mg RE/g, 223.78 ± 29.69 μg/mL), ethyl acetate (72.99 ± 0.05 mg GAE/g, 19.47 ± 0.41 mg RE/g, 294.02 ± 12.70 μg/mL), and petroleum ether (70.29 ± 0.07 mg GAE/g, 4.83 ± 0.05 mg RE/g, 803.64 ± 27.76 μg/mL), respectively. Nonetheless, correlation analysis revealed a moderate relationship between DPPH and TFC. The essential oils from P. macrocarpa seeds demonstrates strong natural antibacterial and antioxidant potential, with hexane as the most efficient solvent. Exploring compounds like 1-(ethenylthio)-octane and 2,2-dimethyl-5-(3-methyl-2-oxiranyl)cyclohexanone may reveal medicinal applications and lead to innovative remedies.

Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique

Antioxidant properties of individual essential oils from lemon (Citrus limon L.), pink grapefruit (Citrus paradise L.), coriander (Coriandrum sativum L.), and clove (Caryophyllus aromaticus L.) buds and their mixtures were studied by capillary gas-liquid chromatography. Antioxidant activity was assessed by oxidation of the aliphatic aldehyde hexanal to the carboxylic acid. The lowest and highest antioxidant activities were exhibited by grapefruit and clove bud essential oils, respectively. Mixtures containing clove bud essential oil also strongly inhibited oxidation of hexanal. Changes in the composition of essential oils and their mixtures in the course of long-term storage in the light were studied. The stability of components of lemon and coriander essential oils in mixtures increased compared to individual essential oils.

The chemical compositions and larvicidal potential against mosquito vectors of selected essential oils obtained from five edible plants were investigated in this study. Using a GC/MS, 24, 17, 20, 21, and 12 compounds were determined from essential oils of Citrus hystrix, Citrus reticulata, Zingiber zerumbet, Kaempferia galanga, and Syzygium aromaticum, respectively. The principal constituents found in peel oil of C. hystrix were beta-pinene (22.54%) and d-limonene (22.03%), followed by terpinene-4-ol (17.37%). Compounds in C. reticulata peel oil consisted mostly of d-limonene (62.39%) and gamma-terpinene (14.06%). The oils obtained from Z. zerumbet rhizome had alpha-humulene (31.93%) and zerumbone (31.67%) as major components. The most abundant compounds in K. galanga rhizome oil were 2-propeonic acid (35.54%), pentadecane (26.08%), and ethyl-p-methoxycinnamate (25.96%). The main component of S. aromaticum bud oil was eugenol (77.37%), with minor amounts of trans-caryophyllene (13.66%). Assessment of larvicidal efficacy demonstrated that all essential oils were toxic against both pyrethroid-susceptible and resistant Ae. aegypti laboratory strains at LC50, LC95, and LC99 levels. In conclusion, we have documented the promising larvicidal potential of essential oils from edible herbs, which could be considered as a potentially alternative source for developing novel larvicides to be used in controlling vectors of mosquito-borne disease.

This paper studies the effect of different extraction procedures such as soxhlet extraction using n-hexane, distillation method and supercritical extraction (SFE) on the physicochemical properties of garlic essential oil. The yield of garlic essential oil by soxhlet extraction, steam distillation and SFE-CO2 methods was approximately 0.441, 0.124 and 0.465 %, respectively. The results of the oil analysis by gas chromatography/mass spectrometry method show the presence of five major compounds, including diallyl sulfide, diallyl disulfide, 3-vinyl-1,2-dithiacyclohex-4-ene, 3-vinyl-1,2-dithiacyclohex-5-ene and diallyl trisulfide. The antioxidant activity of the essential oil obtained by the SFE-CO2 method was significantly higher than by the distillation method, but was lower than the acid ascorbic one.
 Keywords
 Garlic essential oil, SFE, GC-MS, antioxidant activity, extraction.
 References
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The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus nobilis L. by two different distillation methods. The essential oils isolated by hydrodistillation (HD) and steam distillation (SD) were analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Hydrodistillation produced a yield of 0.95 ± 0.06% which is slightly higher than yield obtained by steam distillation 0.79 ± 0.07%. Seventy three compounds in the bay leaves oil obtained by steam distillation were identified while in essential oil obtained by hydrodistillation were identified only 54 compounds. The antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method. Antimicrobial activity of obtained essential oils was evaluated by disc diffusion method in comparison with several chosen antimicrobials. The antimicrobial activity was tested on five microorganisms - Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans. In general, oils produced by steam distillation had higher antimicrobial and antioxidant activities than hydrodistillation extracts. It seems that hydrodistillation is better for higher yield while steam distillation is better to use for more quality oils with stronger biological properties.

Essential oils extraction from Anoectochilus roxburghii using supercritical carbon dioxide and their antioxidant activity

Summary Introduction: Solanum sodomaeum L. has been observed to have several medicinal properties, in particular, in the treatment of several types of human skin cancer. Objective: The influence of the maturation stage of S. sodomaeum fruits on the total lipid contents, fatty acid profiles, essential oil yields and compositions, as well as the antibacterial and antioxidant activities of the essential oils, was investigated. Methods: The fatty acid and essential oil constituents were identified using gas chromatography (GC) and GC–mass spectrometry (GC–MS). The antioxidant properties of essential oil and vegetal oil were assessed using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging and reducing power assays. The antibacterial activity of essential oil was tested using the disc diffusion assay for resistance in human pathogenic bacteria. Results: Mature fruits showed higher total lipid content (17%) and were characterised by polyunsaturated fatty acids (53.87%), represented mainly by linoleic acid (53.11%). Similar yields of essential oils were detected for immature (0.43%) and mature (0.45%) fruits. Tetrahydronaphthalene (41.79%) was detected as the major essential oil component at the immature stage versus dihydrocoumarin pentane (18.27%), hexadecanoic acid (17.43%) and 2-undecanone (13.20%) in mature fruits. The DPPH test showed that essential oils had better antioxidant properties; however, the vegetal oils showed better performance in the reducing power assay. Moreover, the essential oil of S. sodomaeum mature fruits was active against bacterial strains. Conclusions: S. sodomaeum fruits could be a valuable source of natural antioxidants and antibacterial agents.

Evaluation of yield, quality and antioxidant activity of essential oil of in vitro propagated Kaempferia galanga Linn.

In order to investigate the effect of micronutrients foliar application on seed yield and essential oil of black cumin, a field experiment was conducted based on Randomized Complete Block Design (RCBD) with three replications at the farmlocated in Naqadeh city, West Azerbaijan province, Iran, during 2015-2016 growing season. Treatments were separate application of Nano-Iron, Nano-Zinc and Nano-Manganese and double and triple combined treatment of them (Fe+Mn, Fe+Zn, Zn+Mn, Fe+Zn+Mn) and control. Treatments were applied at 2 g/L twice at stem elongation and early flowering stages. Different traits such as plant height, number of follicle per plant, number seed per follicle, 1000- seed weight, biological yield, seed yield, essential oil percentage and yield were measured. The results showed that foliar application of micronutrients had a significant effect on all studied traits and improved yield components and essential oil yield as compared to control. Yield components, seed yield, essential oil percentage and essential oil yield were enhanced by foliar application compared with control. Therefore, combined usage of micronutrients (Nano-Fe+ Nano-Zn+ Nano-Mn) showed the greatest increase in studied traits than their individual usage. The highest seed yield (706.67 kg.ha-1) and essential oil yield (8.66%) were obtained from Fe+Zn+Mn treatment. In general, the results of the present study revealed that the foliar spraying of Nano-micronutrients plays a remarkable role in improving the quantitative and qualitative yield of black cumin as a medicinal plant.

Objectives The objective of this study was to examine the chemical composition and antibacterial and antioxidant properties of essential oil (EOs) and solvent extracts from the leaves of Solanecio gigas , a medicinal plant that has long been used in Ethiopian traditional medicine but has received little scientific attention. Methods Gas chromatography–mass spectrometry (GC–MS) was used to analyse the EOs obtained by hydrodistillation. Soxhlet extraction was used to create solvent extracts using methanol, ethyl acetate, and chloroform. To find the main classes of compounds, phytochemical screening was done. Utilizing the 22-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, antioxidant activity was assessed. The agar diffusion method was used to test the antibacterial activity against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli , and Klebsiella pneumoniae . The microdilution assay was used to determine the minimum inhibitory concentrations (MICs). Colourimetric techniques were used to quantify the total phenolic and flavonoid contents. Results The essential oil contained 15 compounds, as determined by GC-MS analysis, with the main constituents being, Sabinene (38.909%), and α-Pinene (18.20%). The highest levels of flavonoids (130.10 ± 0.08 μg CE/mg) and phenolics (110.2 ± 0.03 μg GAE/mg) were found in the methanolic extract. Compared with the essential oil (IC₅₀ = 10.2 ± 0.22 μg/mL), the methanolic extract showed superior antioxidant activity (IC₅₀ = 2.1 ± 0.11 μg/mL). At concentrations of 25–200 mg/mL, the EOs demonstrated strong antibacterial activity with inhibition zones ranging from 7.1 ± 0.15 mm to 24.1 ± 0.40 mm, especially against K. pneumoniae and E. coli . The MIC range was 80.25 to 250.3 μg/mL. Conclusion The antibacterial and antioxidant qualities of S. gigas essential oil and extracts offer scientific support for the plant's traditional medicinal uses. The EOs demonstrated strong antibacterial activity against both Gram-positive and Gram-negative bacteria, while the methanolic extract, rich in phenolic and flavonoid compounds, showed the strongest antioxidant activity.

The antioxidant activity and free radical scavenging capacity of the essential oil and three different extracts of wildly grown Mentha longifolia (M. longifolia) were studied. The essential oil from M. longifolia aerial parts was isolated by hydrodistillation technique using Clevenger-type apparatus. The extracts were prepared with three solvents of different polarity (n-hexane, dichloromethane, and methanol) using Soxhlet extractor. Maximum extract yield was obtained with methanol (12.6 g/100 g) while the minimum with dichloromethane (3.50 g/100 g). The essential oil content was found to be 1.07 g/100 g. A total of 19 constituents were identified in the M. longifolia oil using GC/MS. The main components detected were piperitenone oxide, piperitenone, germacrene D, borneol, and β-caryophyllene. The total phenolics (TP) and total flavonoids (TF) contents of the methanol extract of M. longifolia were found to be significantly higher than dichloromethane and hexane extracts. The dichloromethane and methanol extracts exhibited excellent antioxidant activity as assessed by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, bleaching β-carotene, and inhibition of linoleic acid peroxidation assays. The essential oil and hexane extract showed comparatively weaker antioxidant and free radical scavenging activities. The results of the study have validated the medicinal and antioxidant potential of M. longifolia essential oil and extracts.

The primary objectives of this study were to investigate how different solvent types and solvent ratios affect both the extraction yield and quality of essential oil derived from coffee beans, with the ultimate goal of optimizing the process for higher yields and superior quality essential oil. This research was conducted in the Research Laboratory of the Chemical Engineering Study Program, where carefully weighed coffee bean samples served as the experimental material. Extraction was performed using two different solvents: 96% ethanol and n-hexane. The Soxhlet extraction technique was employed for a period of three hours, ensuring thorough extraction of the essential oils from the coffee beans. Following extraction, the mixture of coffee and solvent was subjected to distillation in order to separate and remove the solvent, thereby isolating the concentrated essential oil. Subsequent evaluation of the extracted essential oil included determining the percent yield, assessing organoleptic properties, and performing oil component analysis through Gas Chromatography Mass Spectrometry (GC-MS). The findings demonstrated that 96% ethanol provided a substantially higher extraction yield compared to n-hexane. Notably, the essential oil obtained using n-hexane appeared yellow to brown in color and possessed a very weak to weak coffee aroma. In contrast, the oil extracted with 96% ethanol exhibited a deep black color along with a slightly strong to strong coffee aroma, suggesting a richer and more desirable flavor profile. Furthermore, GC-MS analysis revealed that the total peak area of oil components extracted with 96% ethanol was 21.04%, whereas that extracted with n-hexane was only 0.95%. This pronounced disparity clearly highlights the superior efficiency of ethanol as a solvent for extracting high-quality essential oils from coffee beans. These results underscore the significant impact of solvent selection on both the yield and quality of coffee bean essential oil. Ethanol, in particular, shows promising potential for industrial applications in the production of coffee flavorings and fragrances. This study provides valuable insights into optimizing essential oil extraction processes and facilitating the enhanced utilization of coffee bean towards various commercial purposes. Key words: Solvent, Coffee, Ethanol, N-Hexane, GC-MS, Oils, Solvents, Soxhlet, Industry

Com o objetivo de avaliar o comportamento agronômico, o potencial de rendimento de óleo essencial e identificar os quimiotipos de 16 acessos de Lippa alba (erva cidreira) da coleção da Universidade de Brasília foi conduzido um experimento de campo em Latossolo Vermelho, sob irrigação por gotejamento na região do Cerrado do Distrito Federal. Foram avaliados a época de florescimento, hábito de crescimento, área foliar, comprimento da haste, massa fresca de folhas e hastes, massa foliar seca, teor e rendimento de óleo essencial e perfil aromático. O óleo essencial foi extraído por hidrodestilação em aparelho de Clevenger modificado e os constituintes identificados e quantificados através de cromatografia em fase gasosa acoplada a espectrometria de massas. O delineamento experimental utilizado foi de blocos ao acaso, com três repetições, contendo três plantas úteis por parcela. Foram identificados os quimiotipos citral-limoneno, citral-mirceno, limoneno-carvona, citral, linalol, mirceno e linalol-limoneno. Os acessos apresentaram teores máximos de linalol de 89,8% (L.16); mirceno de 47,6% (L.37); limoneno de 36,0%, carvona de 46,9% (L.27) e citral de 56,7% (L.17). Os genótipos com maiores áreas foliares e maiores comprimentos de hastes tenderam a apresentar maiores teores de óleo e maior concentração de linalol. A concentração de óleo foi inversamente proporcional à produção de massa foliar seca. Os acessos que apresentaram os maiores rendimentos dos compostos majoritários do óleo essencial foram: L.16 (0,77 g pl-1), como fonte potencial para a produção de linalol; L.17 (0,17 g pl-1), como fonte de mirceno; L. 27 (0,17 g pl-1), como fonte de limoneno e de carvona (0,21 g pl-1) e L.38 (0,24 g pl-1), como fonte de citral.