Abstract
Background: The aim of this study was to evaluate the performance of three tests for the early pregnancy diagnosis in ewes and does. Methods: A total of 50 ewes and 50 does urine samples were collected in which 25 ewes and 25 does were pregnant, and 25 ewes and 25 does were non-pregnant from different farmers in Babugonj upazila of Barishal district and Sadar upazila of Bhola district. The pregnant and non-pregnant ewes and does were primarily selected from farmer’s record of management history. The pregnancy of ewes and does were confirmed through scanning by B-mode ultrasound machine. Three pregnancy diagnostic tests like barium chloride, copper sulfate and ballottement were evaluated considering B-mode ultrasound technique as gold standard. Results: The sensitivity of manual ballottement technique (MBT) was highest among the three tests: 96% in ewes and 92% in does. On the other hand, the specificity (96%) of the MBT was higher in ewes and that of the copper sulfate test (CST) (91.7%) was higher in does. The highest positive predictive value (PPV) of MBT (95.8%) was in ewes but the PPV of CST was highest (91.7%) in does. The negative predictive value of the MBT was highest both in ewes (92.3%) and does (95.7%). Conclusions: MBT can be considered as a screening test for the early diagnosis of pregnancy in ewes and does. MBT positive cases can be confirmed by ultrasonograpy whenever needed.
Highlights
The people of Bangladesh are loving animals from very old years for domestic as well as companion animals
Determination of progesterone in blood and milk was based on radio immune assay (RIA) and enzyme-linked immunasorbent assay (ELISA) test (Pieterse et al, 1990)
On the other hand specificity (96%) the manual ballottement technique (MBT) was higher in ewes and that of the copper sulfate test (CST) (91.7%) was higher in does
Summary
The people of Bangladesh are loving animals from very old years for domestic as well as companion animals. Laboratory methods like copper sulfate test, Sodium hydroxide test, Barium chloride test, sodium benzoate test are used in different farm animals infrequently. The aim of present study is to evaluate the performance three tests for the early pregnancy diagnosis in pregnant ewes and does. The aim of this study was to evaluate the performance of three tests for the early pregnancy diagnosis in ewes and does. Three pregnancy diagnostic tests like barium chloride, copper sulfate and ballottement were evaluated considering B-mode ultrasound technique as gold standard. Conclusions: MBT can be considered as a screening test for the early diagnosis of pregnancy in ewes and does.
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