Evaluation of the Impact of PGRs in Bean Sprouts Extract on Vegetative Growth, Total Phenolics, and Antioxidant Activity of Chili Peppers, Soybeans, and Potatoes

Indonesia has abundant horticultural commodities, including chilli plants, which have high economic value. However, their productivity remains low at 8.35 tons/ha, compared to the potential 20–40 tons/ha. To boost the productivity of red cayenne pepper, additional supplements such as phytohormones auxin, gibberellin, and cytokinin are needed. This study uses natural sources of phytohormones derived from banana stems and mung bean sprouts. The aim is to measure the antioxidant activity and total phenolic content of various combinations of these natural plant growth regulators (PGRs) to support plant productivity. An experimental design was used, analyzing antioxidant activity using the DPPH method with IC50 values, and total phenolics using the Folin-Ciocalteu method. Six variations were tested: A (banana stem extract 10:0), B (banana stem:bean sprouts 8:2), C (6:4), D (4:6), E (2:8), and F (bean sprout extract 10:0). Antioxidant activity and total phenolics were measured using a UV-Vis spectrophotometer. The results showed that variation C (6:4) had the highest total phenolic content (8.8 mg GAE/g) and an IC50 value of 210 ppm, indicating moderate antioxidant activity. These findings suggest that the combination of banana stem and bean sprout extracts in the right proportion can enhance the functional properties of natural PGRs, potentially supporting the growth and productivity of red cayenne pepper plants.

<p>The effects of dried oregano leaves (Mediterranean and Mexican oregano) extracted using different extraction techniques, solvent types, and six different ratios of each solvent to distilled water on total phenolic (TP) content and antioxidant properties were examined. The Folin-Ciocalteu and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assays were performed to assess the antioxidant capacity. The different species of oregano had a significant effect on TP content (107.6 vs. 34.5 mg GAEg<sup>-1</sup> in Mexican vs. Mediterranean oregano, respectively) (<em>P</em><0.05). Comparing extraction techniques, the vortex procedure significantly increased the measured TP content compared to sonication or shaking (<em>P</em><0.05); however, its effectiveness was sample species and solvent type dependent. Solvent type also had a significant impact on TP content of extracts in decreasing order of acetone, methanol, ethanol, and water (<em>P</em><0.05). The solvent:water ratio on TP content of each extract was significant (<em>P</em><0.05); higher TP content was measured for 40:60 and 60:40 acetone:water ratios for Mediterranean and 60:40 and 80:20 acetone:water ratios for Mexican oregano. The antioxidant capacity had a strong relationship with total phenolic contents. The current findings indicated that the species, extraction techniques, solvent type and the ratio of solvent:water had a significance influence on the TP content of two different species of dried oregano leaf, which may be a possible reason behind most variability reported on TP compounds of herbal and medicinal plants.</p>

This study aims to determine the effect of the combination of NPK fertilizer and bean sprout extract (BSE) on the growth and yield of cauliflower plants and to determine the right dose of the combination of NPK fertilizer and bean sprout extract, so as to provide maximum results on the growth and yield of cauliflower plants in the lowlands. The experiment was carried out in Pusakanagara District, Subang Regency, West Java Province. With an altitude of 15 m above sea level, the air temperature ranges from 270C–300C. The experiment was carried out from October to December 2022. The study used an experimental method and a randomized block design with four treatments and six replications. The treatment consisted of P0: 5 g NPK (as control), P1: 5 g NPK + 75 g/L, P2: 5 g NPK + 100 g/L, P3: 5 g NPK + 125 g/L. The results showed that all treatments of a combination of NPK fertilizer and bean sprout extract had a significant effect on plant height (14 and 28 days after planting(DAP)), number of leaves (14, 28, and 35 DAP), leaf diameter (14 and 35 DAP), flower weight and flower diameter. For the P3 treatment, the combination dose of 5 g of NPK fertilizer and 125 g/l of bean sprout extract had a significant effect and showed the highest yield on plant height, number of leaves, and the highest weight of cauliflower, namely 379.25 g/plant. It can be concluded that the administration of bean sprout extract (BSE) was proven to increase the growth and yield of cauliflower varieties PM 126F. Doses of bean sprout extract fertilizer up to 125 mg/L of water are considered quite effective in efforts to increase the growth and yield of flower cabbage variety PM 126F.

Elephant foot yams make a significant contribution to diets in tribal people of India. However, there is insufficient study of their nutritional and antioxidant value. In this paper the various traits of eleven cultivars of elephant foot yam: BCA-1, BCA-2, BCA-4, BCA-5, BCA-6, NDA-4, NDA-5, NDA-9, IGAM-1, AC-28 and Gajendra were studied and observed during the growth and development stage. The cultivar of BCA-6 contained the maximum amount of starch and total phenol at 100 Days After Planting (DAP) while cv., NDA-9 and NDA-5 showed the maximum content of starch and total phenol at 250 DAP respectively. However, the cultivar BCA-1 stored the maximum amount of carbohydrate at 100 DAP whereas ascorbic acid and β-carotene content was highest at 250 DAP. The protein amount was maximum in cv., BCA-2 and AC-28 at 100 and 250 DAP respectively. This information will provide breeders with the ability to develop desirable types of elephant foot yams having high yields and better nutritional profiles.

One of the techniques that can be applied to overcome seed decline is the invigoration technique using organic materials as priming, namely with young coconut water, bean sprout extract, and aloe vera extract. This study aims to determine the effect of the use of young coconut water, bean sprout extract and aloe vera extract on the viability and vigor of expired cayenne pepper seeds. This study was conducted at the Seed Laboratory of the Agrotechnology Department, Bangka Belitung University. This study used a Completely Randomized Design (CRD) treatment. The treatment given was in the form of organic materials used for priming (Z) with 4 levels of treatment, namely (z0) control, (z1) young coconut water with a concentration of 15%, (z2) bean sprout extract with a concentration of 80%, and (z3) aloe vera extract with a concentration of 50%. Soaking expired cayenne pepper seeds is done for 1 hour. The results of this study indicate that expired cayenne pepper seeds soaked in young coconut water with a concentration of 15% (z1) can increase the parameters of maximum growth potential, seed germination rate, vigor index, plumule length, and dry weight of normal seed sprouts which are better than the control although not significant. The treatment of soaking expired cayenne pepper seeds with organic materials had no significant effect, thought to be due to inaccuracy in the soaking dosage, soaking duration, and abscisic acid content in one of the materials used. Key words: Expired Seeds, Invigoration, Natural Growth Regulator

Amelioration of physiological disorders in pistachio nuts by organic manure and gypsum

The target of the present study was to determine the effect of nitric oxide (NO) on drought stress amelioration in soybean plant. Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10, and 15%) without or with NO (100 μM). Based on our results, drought stress significantly decreased growth in soybean plants. Increase in hydrogen peroxide, malondialdehyde, and aldehyde content indicated drought-induced oxidative stress in soybean plants. Drought stress enhanced the activities of catalase, ascorbate peroxidase, accumulation of proline and glycine betaine, and lipoxygenase activity as well as total phenol and tocopherol content. NO had a beneficial effect on drought tolerance and promoted growth in soybean plants. NO treatment maintained soybean against drought-induced oxidative hurt, thereby improving the antioxidant defense mechanism (enzymatic and non-enzymatic antioxidants). NO application caused osmotic adjustment by up-regulation accumulation of compatible solutes in stressed plants. Enhanced plant growth was linked with induction of phenylalanine ammonia-lyase and tyrosine ammonia-lyase activity and decrease in electrolyte leakage by NO application. Our results revealed that NO had ability to alleviate the destructive effects in soybean plants under drought stress.

Organic farming is now increasingly gaining attention as a solution to the challenges of environmental damage and the need for healthy food in the modern era. One important aspect of organic farming practices is the use of natural Plant Growth Regulators (PGRs) to increase crop yields and quality. This study aims to examine the potential combination of red onion and bean sprout extracts as natural PGRs through total phenolic analysis and antioxidant activity as a reference basis for further application to potato plants. Extracts from both materials were obtained through the maceration method, then formulated in six different ratio comparisons and analyzed for their total phenolic content using the Folin–Ciocalteu method and their antioxidant activity through the DPPH test. The results showed that variation B (8 g/L red onion + 2 g/L bean sprouts) had the highest total phenolic content of 14.77 mg GAE/g and the strongest antioxidant activity with an IC50 value of 173.7 ppm. The results were supported by the DMRT test, which showed significant differences between the combination variations. The content of natural hormones such as auxin, gibberellin, and cytokinin in both materials works synergistically in stimulating the biosynthesis of secondary metabolites such as phenolics, which contribute to antioxidant activity and its effectiveness as a PGR. Thus, variation B has the highest potential to be developed as an environmentally friendly and sustainable natural PGR to support the growth and resistance of potato plants. This formulation shows promising potential for further development in the field of organic farming and nature-based agricultural innovation.

In order to examine how carbon and nitrogen status of a macrophyte may affect its total phenolics (TP) production, the contents of free amino acids (FAA), soluble carbohydrate (SC) and TP were examined in leaves of seven submersed, four floating-leaved, and four emergent macrophytes. The floating-leaved and emergent macrophytes had much higher contents of SC and TP than the submersed macrophytes. The contents of FAA were not significantly different among the submersed, floating-leaved, and emergent macrophytes. Correlations among the contents of FAA, SC, and TP indicated that the production of TP was more dependent on the SC content than on the FAA content.

Senna didymobotrya has been used in Kenya by the Kipsigis community to control malaria as well as diarrhoea. The Pokot prepare charcoal from the stem for milk preservation. Research has not been done to investigate the effect of different extraction solvents on yield, total phenolic and flavonoid content of Senna didymobotrya plant roots. The aim of this study was to compare root extract yield of diethyl-ether, methanol, and aqueous solvents; phytochemical screening; and total phenolic and flavonoid content of Senna didymobotrya plant roots. Extraction was done by the Soxhlet method. Phytochemical screening was done using Harborne's (1973) method with a slight modification. Total flavonoid content was determined by aluminium chloride colourimetric assay at 420 nm. Total phenolic content was determined by Folin–Ciocalteu at 760 nm using UV-Vis spectrophotometry. Extraction yield of diethyl ether, methanol, and distilled water were 3.72 g (7.44%), 4.97 g (9.94%), and 9.09 g (18.18%), respectively, showing a significant difference (p < 0.05) in the yields obtained using the different solvents. Phytochemical screening was positive for phenols, tannins, saponins, gladiac glycosides, anthraquinones, alkaloids, and flavonoids. Total flavonoid content was found to be 48.3 ± 1.5 (QEmg/g) and total phenol content was calculated as 34.5 ± 0.1 (GAEmg/g). Distilled water can be utilized as the best extraction solvent. Senna has a high amount of flavonoid and phenolic content. The limitation of this research is that it only tested root extracts and not leaves, flowers, or seeds. More studies need to be done to isolate the different compounds identified.

BackgroundEnvironmental factors have profound effect on quantity vis-a-vis quality of phytochemicals in medicinal plants. Solanum xanthocarpum Schrad. and Wendl. is among the 10 dashmool species which is utilized in more than hundreds of Ayurvedic preparations including ‘Dashmoolarishta’. Phenolics are the pharmacologically valuable compounds. Therefore, the present study was undertaken to assess the total phenolic (TP) and Caffeic acid (CA) contents in four different plant parts i.e., leaves, fruits, stem and roots of S. xanthocarpum sampled randomly from different locations of Madhya Pradesh, a central Indian state.MethodsPlant samples were collected from 99 places of 29 districts falling in 11 agroclimatic regions of Madhya Pradesh through random sampling. UV-VIS spectrophotometer and HPTLC were used to determine TP and CA contents, respectively. Phytochemical screening was carried out using standard methods.ResultsPreliminary phytochemical screening indicates the presence of alkaloids, cardiac glycosides, flavonoids, phenols, steroids and terpenoids in all plant parts. Quantification of TP and CA contents revealed that both varied significantly between agroclimatic zones as well as within plant parts of S. xanthocarpum. Results revealed that among analysed plant parts, roots and stem harbored highest content of CA while fruits and leaves had the highest TP content. Among agroclimatic regions, accessions of Satpura plateau can be considered rich in CA and TP contents for fruits (0.030%; 28.70 mg CE/g), leaves (0.058%; 27.90 mg CE/g) and roots (0.161%; 5.17 mg CE/g). For stem, highest CA (0.100%) and TP (13.23 mg CE/g) contents were observed in samples of Malwa Plateau and Central Narmada Valley, respectively.ConclusionWe conclude that agroclimatic regions have significant effect on studied phytochemicals and Satpura plateau agroclimatic zone may be targeted for conservation and sustainable utilization of this valuable dashmool species if the target plant parts are fruits, leaves and roots. While, Malwa Plateau and Central Narmada Valley zones may be targeted for stem. Further, fruits and roots may be utilized for extraction of TP compounds and CA respectively.

A pot experiment was carried out to study the ameliorative role of foliar application of an extract of the halophyte Arthrocnemum macrostachyum (A. macrostachyum) on soybean (Glycin max L.) plants grown under salinity stress (0, 75, and 150 mM NaCl). Growth traits, content of photosynthetic pigments, osmolytes, ascorbic acid and total phenol, oxidative damage, and proteins pattern were determined. Growth parameters of soybean plants grown under both saline regimes (75 and 150 mM NaCl) were reduced except for the response to the foliar application of A. macrostachyum extract in either stressed or non-stressed plants. Foliar application of A. macrostachyum extract reinforced the photosynthetic pigment, soluble sugars, and soluble proteins content. Besides, it also minimized the negative impacts of salinity on soybean plants by decreasing the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The results herein showed that the plant water extract and their interactions in the two NaCl levels increased the proline, total free amino acids, total phenols, and ascorbic acid content. Protein patterns in soybean leaves indicated protein expression suppression in soybean plants irrigated with 150 mM NaCl. The magnitude of suppression caused by the saline level was enhanced by using A. macrostachyum extract. The findings of this study indicate that the use of an extract of the halophyte A. macrostachyum can be considered as an unconventional and novel tool in the mitigation of salinity stress.

Abstract. Sidhiq DF, Subositi D, Widiyastuti Y, Pujiasmanto B, Yunus A. 2020. Morphological diversity, total phenolic and flavonoid content of Echinacea purpurea cultivated in Karangpandan, Central Java, Indonesia. Biodiversitas 21: 1265-1271. Purple coneflower (Echinacea purpurea (L.) Moench) is a medicinal plant introduced from North America. While it has been introduced to Indonesia long several decades ago. E. purpurea has not been developed as a raw material for herbal medicines in Indonesia as it faces constraints, namely non-uniform production and lack of information on the total phenolic and flavonoid content if cultivated in the country. This study aims to determine the morphological diversity, total phenolic and flavonoid content of three accession E. purpurea cultivated on lowland area (i.e. 493 m asl) in Tegalgede, Karangpandan, Central Java, Indonesia. This study used a Randomized Complete Group Design (RCBD) method with 1 factor, namely 3 accessions of E. Purpurea: A1, A2, A3 each with four replications. Morphological observations, as well as laboratory analysis, were done in this research to collect quantitative and qualitative data. The quantitative data were analyzed using SPSS to test the difference. We found there is significantly different results E. Purpurea accession in term of plant height, wet stover weight, number of leaves, root weight, root length, number of branches. Quantitative observation in the form of extract yield, total phenol content and total flavonoid highest content of extract residue produced by Accession 3 with 7,655%, while the highest phenolic was produced by Accession 1 with 507,619 mg GAE.g -1 extract, and the highest flavonoid was produced by Accession 2 with 313,869 mg QE.g-1 extract. This study concludes that there is morphological diversity of E. purpurea cultivated in Karangpandan with a significant correlation of accession to residue extract, phenolic, and flavonoid content.

<p>Elephant foot yams make a significant contribution to diets in tribal people of India. However, there is insufficient study of their nutritional and antioxidant value. In this paper the various traits of eleven cultivars of elephant foot yam: BCA-1, BCA-2, BCA-4, BCA-5, BCA-6, NDA-4, NDA-5, NDA-9, IGAM-1, AC-28 and Gajendra were studied and observed during the growth and development stage. The cultivar of BCA-6 contained the maximum amount of starch and total phenol at 100 Days After Planting (DAP) while cv., NDA-9 and NDA-5 showed the maximum content of starch and total phenol at 250 DAP respectively. However, the cultivar BCA-1 stored the maximum amount of carbohydrate at 100 DAP whereas ascorbic acid and β-carotene content was highest at 250 DAP. The protein amount was maximum in cv., BCA-2 and AC-28 at 100 and 250 DAP respectively. This information will provide breeders with the ability to develop desirable types of elephant foot yams having high yields and better nutritional profiles.</p>

The aim of the current work is to determine the main biologically active substances and antioxidant activity of aqueous-ethanolic extracts of cultivated rosehip fruit obtained by ultrasonic treatment. The extraction was performed under the following technological parameters: ultrasound frequency 37 kHz, solvents 50 and 70% ethanol, raw material: solvent ratio = 1:20, temperature 20, 40 and 60°C, duration 15, 30 and 60 min. Tannins, ascorbic acid and total phenolic contents were determined in the extracts. The highest tannin content (1.90%) was obtained by extraction with 50% ethanol, temperature 60°C and process duration 60 min. The total phenolic content under the different process conditions is comparable. Higher values of total phenols (45.43 mg GAE g-1) were determined at 50% ethanol solvent, 40°C temperature and 60 min process duration. The highest values of antioxidant activity, by the DPPH method, were reported in extracts with 50% ethanol, temperature 40°C and duration 60 min (422.5 μmol TE/g), and by FRAP method under the same extraction conditions - the value was almost twice lower (231.9 μmol TE).