Evaluation of the immune response against Brucella abortus in splenectomized mice

Abstract

Event Abstract Back to Event Evaluation of the immune response against Brucella abortus in splenectomized mice Alice B. Rezende1*, Náira N. Neto1, Otávio H. Martins Assis1, Carolina S. Miranda1, Marcela Mota1, Gilson C. Macedo1 and Henrique C. Teixeira1 1 Universidade Federal de Juiz de Fora, Brazil Introduction: The influence of the spleen in control of intracellular bacterial infections remains unclear. Brucellosis is a zoonosis caused by the facultative intracellular bacteria of the genus Brucella. Thousands of new cases of human brucellosis are diagnosed each year worldwide. In Brazil, bovine brucellosis is endemic throughout the country, compromising the quality of cattle. Brucella abortus has a tropism for the spleen and induces an immune response mediated by cytokines such as IFN-y and TNF-α. The objective of this work was to investigate the immune response against Brucella abortus in splenectomized mice. Methods and Results: C57Bl/6 mice were divided into a sham-operated control group (SH) and splenectomized group (SP). Thirty days after surgery, animals were intraperitoneally infected with 106 bacteria/mouse of Brucella abortus (S2308). Twenty one days after infection, B. abortus colony-forming units (CFU) were evaluated in macerated liver. Cytokine production (IFN-y, IL-12, TNF-α, IL-6, IL-10) was analyzed in liver (ELISA). A higher number of B. abortus CFU was observed in liver of splenectomized mice (Ln = 3.8 ± 0.1) in comparison to the sham-operated group (Ln = 3.4 ± 0.1). IFN-y and IL-12 production was lower in liver of SP mice (600.3 ± 75.9 and 1303 ± 99.3 pg/ml, respectively). In addition, SH animals presented a greater production of TNF-α, IL-6 and IL-10 in liver in comparison to the splenectomized group (p<0.05). Conclusion: Splenectomy reduces the capacity of C57Bl/6 mice to control B. abortus infection which correlates with a lower cytokine production in splenectomized mice. Financial support: CNPq, FAPEMIG, CAPES. Acknowledgements Financial support: CNPq, FAPEMIG, CAPES. Keywords: Spleen, Splenectomy, Immune responses, Intracellular bacterial infections, Brucella abortus Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Rezende AB, Neto NN, Martins Assis OH, Miranda CS, Mota M, Macedo GC and Teixeira HC (2013). Evaluation of the immune response against Brucella abortus in splenectomized mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00313 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Alice B Rezende, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil, alicerezende.ufjf@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alice B Rezende Náira N Neto Otávio H Martins Assis Carolina S Miranda Marcela Mota Gilson C Macedo Henrique C Teixeira Google Alice B Rezende Náira N Neto Otávio H Martins Assis Carolina S Miranda Marcela Mota Gilson C Macedo Henrique C Teixeira Google Scholar Alice B Rezende Náira N Neto Otávio H Martins Assis Carolina S Miranda Marcela Mota Gilson C Macedo Henrique C Teixeira PubMed Alice B Rezende Náira N Neto Otávio H Martins Assis Carolina S Miranda Marcela Mota Gilson C Macedo Henrique C Teixeira Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.