Abstract
Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses.
Highlights
It is known that one of the important ensuring the maximum yield of potatoes is the planting material not infected with viruses and bacteria [1].When potato plants are affected by pathogens, tubers are formed in the future, which are significantly inferior to healthy ones in terms of basic quality indicators [2].To date, the production of healthy seed material is provided through an effective direction – the method of improving the apical meristem, which at the same time is associated with certain difficulties.The problem of the method is that the isolated small explants are free of viruses, but they do not regenerate well, and large explants are simultaneously affected by viral and bacterial infection during further cultivation
The production of healthy seed material is provided through an effective direction – the method of improving the apical meristem, which at the same time is associated with certain difficulties
The purpose of the work was to study the effectiveness of improving the seeds of promising potato varieties, which provides an increase in the number of virus-free regenerating plants with the combined use of the method of apical meristems and in vitro improvement on optimized nutrient media
Summary
The problem of the method is that the isolated small explants are free of viruses, but they do not regenerate well, and large explants are simultaneously affected by viral and bacterial infection during further cultivation. These factors undoubtedly influence the yield of healthy meristems [3,4]. The use of biotechnological methods in the laboratory for the purpose of improving promising potato varieties, as well as increasing the rate of microclone reproduction have pronounced advantages compared to clones intended for selection, which affects the reduction of time for obtaining healthy plants, and as a result, ensuring the production of mini tubers in sufficient quantity
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