Abstract
The Bone morphogenetic protein receptor-1B (BMPR-1B) was one of the most major candidate gene for sheep follicular development and litter size trait. The BMPR-1B gene encoded a structural and functional highly conserved transmembrane protein related to sheep reproductive performance. To further validate its genetic effect, genotype-phenotype associations were performed in Tibetan sheep. Through DNA sequencing of the entire coding region of the BMPR-1B gene using DNA of 30 unrelated Tibetan ewes (10 Singletons Tibetan ewes, and 20 Twins Tibetan ewes). And genotyping the target SNPs loci using PCR-RFLP (or PCR-SSCP) combined with DNA sequencing and analyzing their association with lambing traits in 510 Tibetan ewes. One mutation in 3′UTR (A1354G) and three mutations in exons (G192A, A746G, T864C) were found to be strongly associated with lambing traits in Tibetan ewes in this study. χ2 suitability test showed that all of SNPs were Hardy-Weinberg equilibrium. After independence test, A746G, T864C and A1354G of BMPR-1B gene polymorphisms were significantly correlated with litter size in all-types of Tibetan sheep. These results indicate that the variations in the BMPR-1B genes and their corresponding genotypes may be considered as marker-assisted selection (MAS) for litter traits in Tibetan sheep breeding.
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