Abstract
Considering the high rates of global antimicrobial resistance, researchers are looking for new ways to deal with the resistance. Therefore, this study is aimed at analyzing the bioactive compounds and antimicrobial effects of honey and alcoholic extracts of Zataria multiflora and Black cardamom. The mixture of honey (30%) and alcoholic extracts of Zataria multiflora (35%) and Black cardamom (35%) as named F6 revealed the antibacterial activity against three tested bacteria ( MIC = 1000 μg/ml). Thymol, resorcinol, and phenol, 2-methyl-5-(1-methylethyl, 3,7-octadiene-2, 6-diol, 2, 6-dimethyl) were identified as potential antimicrobial agents using GC-MS. The F6 toxicity to RBCs and HEK293 human cells was 11.13 and 13.84 times lower for Triton X-100 toxicity (95%), respectively. We concluded that F6 has the best antibacterial activity against E. coli and less toxicity against tested eukaryotic cells due to the presence of chemicals such as hydrogen peroxide, thymol, and resorcinol.
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