Evaluation of the antifungal activity of the purified chitinase 1 from the filamentous fungusAphanocladium album

Abstract

Crude protein preparations from the culture filtrate of the filamentous fungus Aphanocladium album, a hyperparasite of rust fungi, strongly inhibited growth of a strain of the fungus Nectria haematococca pathogenic on pea. Crude protein from the filtrate of the variant E3 of A. album, hyperproducing chitinase, was less inhibitory than crude protein from the filtrate of the wild-type strain E1. The antifungal potential of a purified chitinase from A. album, called chitinase 1, was compared to that of a plant chitinase with known antifungal activity, obtained from pea (Pisum sativum). Although purified chitinase 1 of A. album degraded chitin more completely than did pea chitinase, it did not inhibit growth of N. haematococca, either alone or in the presence of a pea β-1,3-glucanase. Furthermore, chitinase 1 from A. album failed to enhance the antifungal activity of pea chitinase. These results indicate that the extracellular proteins of A. album inhibit growth of some fungi by other means than through their chitinase 1 activity.