Abstract
The suitability of ten direct plating media for isolating and enumerating four strains of uninjured and injured Listeria monocytogenes from dry- and country-cured hams, and raw oysters was evaluated. Five different lots of each food were inoculated with approximately 10 2 ,10 4 , or 10 5 –10 6 viable L. monocytogenes cells g −1 . Populations of the organism were determined by surface plating appropriate dilutions onto the test media. Inoculated media were incubated at 30 °C for 48 h before L. monocytogenes colonies were counted and confirmed. LiCl Phenylethanol Moxalactam Agar (LPMA) was selected as the most suitable medium for isolating and enumerating L. monocytogenes from both types of ham, while Dominguez Rodriguez Isolation Agar (DRIA) was selected as most suitable for raw oysters. Selection of the most suitable media was based on relative recovery of viable cells as well as ease of recognizing and counting L. monocytogenes colonies.
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