Abstract
Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel dogs were used. Bilateral acute full thickness skin wounds (3 cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day 0 and then every week for three consecutive weeks. The left wound was left as control. Wound contraction and epithelization were clinically evaluated. Expression of collagen type I (COLI) A2, (COLIA2),histopathology and immunohistochemical (IHC) staining of COLI α1 (COLIA1) were performed on skin biopsies at first, second and third weeks. The catalase activity, malondialdehyde (MDA) concentration and matrix metalloproteinase (MMP) 9 (MMP-9) activity were assessed in wound fluid samples. All data were analysed statistically. The epithelization percent significantly increased in the PRP-treated wound at week 3. Collagen was well organized in the PRP-treated wounds compared with control wounds at week 3. The COLIA2 expression and intensity of COLIA1 significantly increased in PRP-treated wounds. MDA concentration was significantly decreased in PRP-treated wound at week 3. The catalase activity exhibited no difference between PRP treated and untreated wounds. The activity of MMP-9 reached its peak at the second week and was significantly high in the PRP-treated group. S/C infiltration of autologous PRP at the wound margins enhances the wound epithelization and reduces the scar tissue formation.
Highlights
Skin wounds are the common and frequent purpose for research in veterinary practice
After bilateral induction of full thickness skin wound at the thoracic region, the right sides of each dog were subcutaneously infiltrated with activated Platelet-rich plasma (PRP) and the left sides were left as untreated
The wounds induced at 0 day in both control and PRP treated groups were similar in size (Figure 1a, b)
Summary
Skin wounds are the common and frequent purpose for research in veterinary practice. Healing of skin wounds can be challenging and more than one manner of repair may possibly be fruitful [1].After skin injury, healing begins immediately. Skin wounds are the common and frequent purpose for research in veterinary practice. Healing of skin wounds can be challenging and more than one manner of repair may possibly be fruitful [1]. Three to five days after injury; granulation tissue begins to replace the fibrin plug that fills the wound. The greatest rate of collagen accumulation occurs between 1 and 2 weeks after injury rendering the wound its tensile strength. After 2–3 weeks, the wound begins to mature as collagen content and fibre orientation change [3,4]. Multiple regulators such as growth factors and cytokines, integrins, keratins, matrix metalloproteinases (MMPs), chemokines and extracellular macromolecules regulate these processes [2,5]
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