Evaluation of root dentin microhardness prepared with hand and two rotary file systems with 2.5% sodium hypochlorite irrigation solution - an in vitro study

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Background: Success in endodontic therapy depends on effective chemomechanical preparation of the root canal, but their compromising effects on root dentin microhardness should be taken into consideration. AIM: To evaluate the effect on microhardness of root dentin prepared with hand stainless steel K-Files, ProTaper and K3 NiTi Rotary file systems with 2.5% Sodium Hypochlorite as common irrigating solution. Materials and Methods : Fifty human mandibular single-rooted premolar teeth selected. Access opening was done and working length was determined. All the specimens were then randomly divided into five groups with 10 specimens in each group. Group I- Neither instrumented nor irrigated. Group II- 2.5% NaOCl irrigation Group III- Stainless steel K-files using 2.5% NaOCl Group IV- ProTaper rotary files using 2.5% NaOCl solution. Group V - K3 rotary files using 2.5% NaOCl solution. The specimens were prepared for microhardness testing at a distance of 500 mm and 1000 mm from the lumen of each sample using vickers microhardness test. Results: 1) Significant difference in microhardness values were seen at 500 mm and 1000 mm (P from the lumen in all groups. 2) Statistically significant differences were seen among all the groups except between Group IV & V. Conclusion: Instrumentation with NiTi Rotary files affected dentin microhardness significantly to a lesser extent when compared to Stainless Steel K files using 2.5% NaOCl irrigating solution. Keywords: Microhardness, Sodium hypochlorite, Nickel Titanium Rotary files.,