Abstract
KDR mutations in the sodium channel gene (VGSC) have already been detected in at least 13 species of Anopheles mosquitoes, mostly African species, but the molecular techniques for detection in Latin American species have yet to be determined. In our country, Anopheles darlingi species is the main vector of Malaria, and A. albitarsis, the secondary vector. We used samples of Anoheles from the species A. strodei, A. albitarsis, A. fluminensis, A. evansae, A. nuneztovari, A. nyssorhynchela lutzi and A. oswaldoi collected at the departments of Caaguazu and Alto Parana in Paraguay. For the amplification and sequentiation, primers reported for the VGSC gen of A. strodei in Guatemala were used and were specific only for A. strode in this case. The sequence revealed the TTA codon that codifies for a leucine as the TTG sequence, reported for the susceptible version at position L1014. The amplified fragment is approximately 225 base pairs. To our knowledge, this is the first characterization of the VGSC gene in Anopheles mosquitoes in Paraguay and for the species A. strodei
Highlights
INTRODUCCIÓN La malaria es una enfermedad transmitida a los humanos a través de una picadura de un mosquito hembra del género Anopheles infectado con parásitos del género Plasmodium Se estima que, en 2010, hubo 216 millones de casos de malaria en el mundo y que esta enfermedad causó más de 150,000 muertes(1)
For the amplification and sequentiation, primers reported for the Voltage-gated sodium channel (VGSC) gen of A. strodei in Guatemala were used and were specific only for A. strode in this case
The amplified fragment is approximately 225 base pairs. This is the first characterization of the VGSC gene in Anopheles mosquitoes in Paraguay and for the species A. strodei
Summary
INTRODUCCIÓN La malaria es una enfermedad transmitida a los humanos a través de una picadura de un mosquito hembra del género Anopheles infectado con parásitos del género Plasmodium Se estima que, en 2010, hubo 216 millones de casos de malaria en el mundo y que esta enfermedad causó más de 150,000 muertes(1). ′3)(17).La amplificación del segmento 6 del dominio II del gen VGSC empleando cebadores reportados se llevó a cabo en una mezcla de reacción de 25 μL que contenía 17.55μL de agua.
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