Abstract
Results of enzymatic hydrolysis of pine kraft cellulosic pulps (Kappa numbers ranging from 17.2 to 86.2) and waste fines from paper production line suggest that they are potential feedstocks for biofuel production. Glucose (61–68 % d.w.) and reducing sugars (around 90 % d.w.) yields from the pine cellulosic pulps with Kappa numbers ranging between 17.2 and 47.7 were higher than from the two pulps with Kappa numbers of 60.9 and 86.2. Glucose and reducing sugars yields from the latter pulps were around 54 and 45 % d.w. and 78 and 67 % d.w., respectively. The highest yields of glucose and total reducing sugars on a pine wood dry weight basis (29.3 and 43.1 %, respectively) were derived from the pulp with the Kappa number of 47.7. Thus the complete lignin removal from pine wood was not necessary to obtain maximum yields of glucose and other reducing sugars. Enzymatic hydrolysis of the fines from pine wood processing in a paper mill yielded around 75 % glucose and 78 % total reducing sugars on a dry weight basis that corresponded to around 34 and 36 % on a pine wood dry weight basis.
Highlights
Results of enzymatic hydrolysis of pine kraft cellulosic pulps (Kappa numbers ranging from 17.2 to 86.2) and waste fines from paper production line suggest that they are potential feedstocks for biofuel production
The preparation NS-22086, which was used in this study for hydrolysis of pine cellulosic pulps with Kappa numbers ranging from 17.2 to 86.2, pine woodchips (0.43–0.8 mm) and fines from paper mill, was selected as described by Buzała et al (2015)
Glucose yield from cellulose contained in the woodchips was only 41.8 % d.w. These results demonstrate that the complete lignin removal from wood is not necessary to maximize glucose yields from cellulose
Summary
Results of enzymatic hydrolysis of pine kraft cellulosic pulps (Kappa numbers ranging from 17.2 to 86.2) and waste fines from paper production line suggest that they are potential feedstocks for biofuel production. The highest yields of glucose and total reducing sugars on a pine wood dry weight basis (29.3 and 43.1 %, respectively) were derived from the pulp with the Kappa number of 47.7. Enzymatic hydrolysis of the fines from pine wood processing in a paper mill yielded around 75 % glucose and 78 % total reducing sugars on a dry. Enzymatic degradation of cellulose and hemicelluloses contained in lignocellulosic biomass to simple sugars is not as easy as starch hydrolysis because of the complex, tightly bound and crosslinked, three dimensional structure of plant tissues and protective impact of lignin, resinous substances and waxes. Lignocellulosic biomass must be pretreated before enzymatic digestion to remove lignin and increase the accessible surface area of cellulose. Wood processing begins with comminution to chips, which undergo chemical delignification by the sulfate method, yielding cellulosic pulps of various residual lignin contents. Cellulosic pulps obtained from low quality wood, e.g., partly degraded by microorganisms, may have insufficient characteristics to be used for production of paper because of shortened cellulose fibers
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