Abstract
BackgroundBeing a part of Chinese as well as ayurdic herbal system, roots of Rumex hastatus D. Don (RH) is highly medicinal, used to regulated blood pressure. It is also reported that the plant is diuretic, laxative, tonic, used against microbial skin diseases, bilious complaints and jaundice. The present study is conducted to evaluate phytochemical, antimicrobial, antitumor and cytotoxic activities of extract obtained from R. hastatus roots.MethodsRH roots were powdered and extracted with methanol to get crude extract. Crude extract was further fractioned on the basis of increasing polarity, with n-hexane (HRR), chloroform (CRR), ethyl acetate (ERR), n-butanol (BRR) and residual aqueous fraction (ARR). Methanol extract and its derived fractions were subjected to phytochemical screening and assayed for antibacterial activities via agar well diffusion method. Antifungal activities were checked through agar tube dilution method whereas potato disc assay was employed for the determination of antitumor activity. On the other hand cytotoxic activities were conducted using brine shrimps procedures.ResultsThe results obtained from phytochemical analysis indicate the presence of alkaloids, anthraquinones, flavonoids and saponins in all the fractions. Most of the plant fractions showed substantial antimicrobial activities, which is in accordance with the spacious use of tested plant samples in primary healthcare center. Fractions of R. hastatus roots for cytotoxicity were tested as an effective cytotoxic was found as BRR > MRR > CRR > ARR > ERR > HRR. Ranking order of fractions of R. hastatus roots for effective antitumor screening was found as MRR > BRR > ARR > CRR > ERR > HRR.ConclusionsThese results showed that R. hastatus appeared as an important source for the discovery of new antimicrobial drugs and antitumor agents; verify its traditional uses and its exploitation as therapeutic agent.
Highlights
Being a part of Chinese as well as ayurdic herbal system, roots of Rumex hastatus D
For the treatment of human pathogenic diseases, use of plant extracts or fractions having antimicrobial properties can be of great importance
As reported by World Health Organization (WHO) about 80 % of the world’s population depends mainly on conventional medicines that involve the use of plant extracts [15, 16]
Summary
Being a part of Chinese as well as ayurdic herbal system, roots of Rumex hastatus D. The present study is conducted to evaluate phytochemical, antimicrobial, antitumor and cytotoxic activities of extract obtained from R. hastatus roots. Natural products derived from plant extracts/fractions are novel therapeutic agents for various infectious as well as degenerative diseases. For the treatment of human pathogenic diseases, use of plant extracts or fractions having antimicrobial properties can be of great importance. Food preservation requires evaluation of natural resources such as herbal fractions and isolates with antimicrobial properties as the long historic use of herbs has proved their safety and efficacy in various traditional medicine systems. One of the valuable means used to screen bioactive compounds from plant extracts is brine shrimp lethality assay [5] It is considered as a convenient probe for primary evaluation of toxicity [6]. The present study is arranged to screen and evaluate antibacterial, antifungal, antitumor and cytotoxic activities as well as phytochemical analysis of crude methanol extract
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