Abstract
Ficus palmatais a very potent medicinal and multipurpose plant which has gained global significance due to medicinal and multipurpose utility. It bears deep violet to black berries which are known to human beings from centuries for their effects on health. The plant is wildly distributed throughout Azad Jammu and Kashmir. The phylogenetic relationship among these natural Ficus palmata ecotypes from Azad Jammu and Kashmir is not established so for using reliable molecular markers. ISSR has been proved to be an effective tool for the determination of phylogenetic relationship among closely related species. To construct a population level genetic profile for investigation and exploitation of genetic diversity of Ficus palmata, 25 ecotypes of Ficus palmata in AJK were analyzed using ISSR (Inter simple sequence repeats) markers. Phylogenetic distance estimated revealed that the ecotypes expressed common heritage for their phylogenetic relationship with a considerable genetic diversity among them as well. Quite a few Ecotypes showed close relationship irrespective of their geographic distances and morphological attributes. The research evolved a significant outcome to start a breeding program from the evolution of Ficus palmata varieties for the mountain areas of Azad Kashmir. Keywords: Ficus palmata; ISSR; Ecotypes; Genetic diversity http://dx.doi.org/10.19045/bspab.2017.60031
Highlights
Ficus palmata is a very potent medicinal and multipurpose plant which has gained global significance due to medicinal and multipurpose utility
Cluster analysis of all the amplified fragments produced by 05 intersimple sequence repeat (ISSR) primers Dendrogram generated by NTSYS version 2.1e can be divided into two sub Clusters i.e. sub Cluster1a and sub Cluster 1b.All of the studied ecotypes of Ficus palmata have shown a similarity of 63% (Figure 3)
The results indicated that ISSR markers have been successfully utilized for assessing the genetic diversity and revealed a remarkable molecular discrimination between the Ficus palmata ecotypes under study
Summary
Evaluation of phylogenetic relationship among Ficus palmata forrsk. wild ecotypes from Azad Jammu and Kashmir using inter simple sequence repeats (ISSR). Citation Ghazala Javed, Syed Abdul Majid, Rizwan Taj and Asia Bibi. Evaluation of phylogenetic relationship among ficus palmata forrsk. Wild ecotypes from Azad Jammu and Kashmir using inter simple sequence repeats (ISSR).
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