Evaluation of microRNA and some immunological factors associated with SARS-CoV-2 infection

Abstract

Coronavirus (CoV) is a family of RNA viruses that cause disease in humans and other animals by entering the body via ACE2 receptors found in various organs such as the heart, lungs, kidneys, and digestive system. Coronaviruses are large particle viruses that are enveloped, spherical, have surface protrusion-forming spikes, and have a large positive- sense single-stranded RNA genome. A total 90 samples were collected from people suspected of being infected with SARSCoV-2, 50 blood samples were collected from patients, and 40 samples were collected from healthy individuals as a control group, who aged 20-80 years and carried out during September (2020) to January (2022) at Al-Hussein Teaching Hospital. All were checked by using real- time quantitative polymerase chain reaction (RTPCR) technology. Evaluations of miRNAs were performed in a group of newly infected patients with the SARSCoV-2 virus and healthy individuals who were cured, the result found MiRNA -192 gene have 84 % positive RT-PCR result in comparison with 16 % in which have negative result , while the other gene MiRNA - 31 have 36% positive result in comparison with 64% as a negative result, as well as 100 % of H.K. gene.