Abstract
Two methods, (1) the indirect immunofluorescent antibody technique (IFAT) using head squashes, and (2) the method of tissue trituration and inoculation of supernatant into a susceptible assay system, were evaluated for detection of La Crosse (LAC) virus infection of individual Aedes triseriatus . Twenty-one days after fully engorging on an infectious blood meal containing LAC virus, 63% (119/189) of the control group females transmitted virus to suckling mice; virus infection persisted in 97% (184/189) of the control group females. The test group females were cut into 3 parts (head, anterior thorax, abdomen) to determine the extent of viral infection using IFAT on head squashes and the trituration method on thoracic and abdominal tissues. Head-squash I FAT indicated 71% (52/73) of the test group had disseminated infections; 21% (15/73) had nondisseminated infections. Exclusive use of head-squash IFAT would have indicated a 3 to 24% lower infection rate than the trituration method. However, judicious use of the IFAT will save time and reduce the cost of assay, since only mosquitoes with negative head squashes need be further assayed; in this study a 70% reduction in individual trituration assays would have been realized. A comparison of test and control groups indicated disseminated infections could not be directly equated with the probability of virus transmission.
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