Evaluation of Expression Pattern of Mirna146a in Pakistani Rheumatoid Arthritis Patients

Abstract

Event Abstract Back to Event Evaluation of Expression Pattern of Mirna146a in Pakistani Rheumatoid Arthritis Patients Sami Ullah1*, Peter John1 and Attya Bhatti1 1 National University of Science and Technology, Pakistan Objectives: The objectives of the present study were to identify the role of miRNA146a in rheumatoid arthritis (RA) pathogenesis and to evaluate expression of miRNA146a and its target genes in RA patients. Methods: In the present study, expression of microRNA146a (miRNA146a) and its target genes were analyzed using Real time PCR and Semi-quantitative Reverse transcription PCR in blood and synovial fluid of rheumatoid arthritis patients. Results: An average of 0.7 fold increase was observed in expression of miRNA146a in blood and 14 fold in synovial fluid in rheumatoid arthritis patients as compared to healthy controls. Tumor necrosis factor receptor-associated factor 6 (TRAF6) was down-regulated and its expression correlated with miRNA146a in blood but not in synovial fluid. Interleukin-1 receptor-associated kinase 1 (IRAK-1) and Tumor Necrosis factor alpha (TNF-α) was up-regulated in both blood and synovial fluid. IRAK-1 expression did not correlate with miRNA16a in both blood and synovial fluid while TNF-α correlated with miRNA146a expression in synovial fluid only (R square = 1.4). Conclusion: Increased expression of miRNA146a and its statistically significant correlation with expression of TNF-α means that miRNA146a can be used as a potential diagnostic biomarker in rheumatoid arthritis. Acknowledgements Higher Education Commission of Pakistan is acknowledged for the funds provided to conduct the research Keywords: MicroRNA146a, real time PCR, Rheumatoid arthritis, biomarkers, expression analysis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Ullah S, John P and Bhatti A (2013). Evaluation of Expression Pattern of Mirna146a in Pakistani Rheumatoid Arthritis Patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00037 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Sami Ullah, National University of Science and Technology, Isl, Pakistan, samibiotech@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sami Ullah Peter John Attya Bhatti Google Sami Ullah Peter John Attya Bhatti Google Scholar Sami Ullah Peter John Attya Bhatti PubMed Sami Ullah Peter John Attya Bhatti Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.