Evaluation of effectiveness of low level laser therapy in accelerating orthodontic tooth movement-An in vivo study

Abstract

To assess the acceleration of tooth movement in canine retraction and to assess the interleukin 1β level in Gingival Crevicular Fluid. 0.022 slot MBT brackets, 0.018 inch stainless steel arch wire, Low level laser (Gallium-Aluminium-Arsenide Laser), ELISA kit, Micro-capillary tube, Digital Vernier calliper, Dontrics gauge, NiTi closed coil springs Open labelled split mouth, non-experimental interventional institutional based study. By coin toss method, each of the participant’s mouth was randomly assigned either to the laser therapy (group 1) and 40 reference sites (group 2). On the experimental site orthodontic treatment with fixed appliance, a combination with low level laser therapy (Gallium-Aluminium-Arsenide laser) to the canine on the days 3,7,14 and then every 15 days for 2 months was initiated. Then 5µL of Gingival Crevicular Fluid (GCF) was collected to analyse the interleukin 1β levels using the ELIZA kit. The measurements were made with the help of a digital Vernier calliper. The laser group showed a significant greater reduction in the canine to molar distance than the site treated with conventional technique. The interleukin 1 β levels raised significantly for the site treated with laser as compared to the other. We observed that: Increased inflammatory response in the form of raised interleukin levels and Faster distalization of the canine when adjunct with low level soft tissue laser. The entire data was statistically analyzed using Statistical Package for Social Sciences (SPSS version 21.0, IBM Corporation, USA) for MS Windows. In Statistics, unpaired t-test is a used to compare equality of two population means and F-test is used to compare the equality of two population variance using that the samples were drawn from different populations. There was a clinically appreciable amount of retraction seen resulting in faster space closure between the canine and the first permanent molar in the group that had low level laser than the other group that was treated by the conventional therapy. We also observed a considerable rise in the interleukin 1 β levels in the laser group.