Abstract
A novel class of Mn(III) and Fe(III) complexes of L-tyrosine-based ligand has been synthesized and characterized through various analytical and spectroscopic techniques. These complexes were found to exhibit efficient binding properties with the biomolecules viz. calf thymus DNA and BSA. The ability of complexes to bind with such biomolecules has been explored through absorption, emission and viscosity measurements. Based on spectroscopic techniques we can conclude that the complexes could bind to DNA via intercalation. It was observed that these complexes can cleave pBR322 DNA in gel-electrophoresis technique through oxidative mechanism. The BSA was quenched by the complexes around 340 nm adopting a mechanism of static mode. The binding constants, thermodynamic parameters and the donor to acceptor distance were calculated. Besides, molecular docking simulations were carried out for the complexes with human DNA topoisomerase and BSA protein. The docked poses are visualized to provide supportive evidence to the interaction of the synthesized complexes with DNA/BSA.
Highlights
Biomolecules such as proteins or nucleic acids are interesting as the target since they act as the key molecules in the metabolic pathways associated with a specific disease.[1]
The schematic representation of the Schiff Base ligand synthesized from the effective condensation of 9,10-phenanthrenequinone and L-tyrosine is shown in Scheme 1 and Scheme 2 represents the complexation of the ligand with Mn(III)/Fe(III) ions
The interaction of the complexes with bovine serum albumin (BSA) was studied at three varying temperatures and observed that the fluorescence quenching process may be accomplished as static quenching
Summary
Biomolecules such as proteins or nucleic acids are interesting as the target since they act as the key molecules in the metabolic pathways associated with a specific disease.[1]. Interactions of small metal complexes with DNA and proteins are the key research areas of current years as there are enough potentials of development of new therapeutic agent showing antitumor properties and possibility of the transportation of these molecules throughout the physiological system via protein binding.[5,6,7,8] The metal complexes–nucleic acid interaction has gained the attention of researchers due to their excellent biological activity. Albumin binding affects the pharmacokinetics, trafficking and efficacy of metal-based therapeutics.[17] Amino acids are small molecules capable of metal complexation via amino and carboxylate groups.[18] It has been reported that transition metal complexes of amino acids could possess better fungicidal, anti-bacterial, antiviral and anti-tubercular activities.[19] we report the synthesis of Mn(III) and Fe(III) complexes of L-tyrosine-based ligand and their interaction with CT-DNA/BSA using various spectral methods
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