Abstract
developed for quantifying CMV DNA in immunocompromised patients Results: The detection limit of TaqMan polymerase chain reaction assay for CMV-DNA was 10 genome per reaction and the linear measure interval was 1 to 107 copies per reaction (r2 = 0.999). The reproducibility of the TaqMan assay was initially evaluated by interassay (between-runs) and intraassay (within-run). The interassay and intraassay coefficients of variation were 9.79% and 10.85%, respectively. The specificity of the assay was determined among herpesviridae subfamily. No positive signals were detected. Initial application of the quantitative real-time PCR to serum of infant indicated the effective of assay for CMV quantitation with 100% sensitivity (n = 5). Conclusion: In-house TaqMan assays may potentially serve as a useful tool for rapid quantification of CMV infections in immunocompromised patients.
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