Abstract
Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34IU/g) and AMCQS1-9 (36IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903IU/μg) and Schizochytrium AMCQS1-9 (1.44IU/μg).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
