Abstract

Objective: To evaluate the bactericidal activity of an antiseptic gel for hygiene and disinfection of the hands by rubbing.
 Methods: The dilution-neutralization method was used according to Colombian Technical Standard NTC, 2009, 12, 16 in Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC), 5 strains, over 6 different types and with 6 replicas per time were exposed to the antiseptic gel, using Letheen Broth as a neutralizer substance.
 Results: A 99% of reduction was obtained with Staphylococcus aureus 6538, Enterococos hirae 10541, Pseudomonas aeruginosa 15442 and Klebsiella sp, within the first 30 s of exposure to the gel and within the first 60 s of exposure using the Escherichia coli 19538. All strains used coming from American Type Culture Collection (ATCC).
 Conclusion: It was confirmed that the product is effective. In presence of a neutralizer substance, the microorganisms were not inhibit, so the growing reduction occurs due to the action of the product.

Highlights

  • Most of the infections present in hospitals and medical facilities are linked with poor hygiene practices at the moment of patience assistance, this is known to be a public health issue

  • The dilution-neutralization method was used according to Colombian Technical Standard NTC, 2009, 12, 16 in Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC), 5 strains, over 6 different types and with 6 replicas per time were exposed to the antiseptic gel, using Letheen Broth as a neutralizer substance

  • A 99% of reduction was obtained with Staphylococcus aureus 6538, Enterococos hirae 10541, Pseudomonas aeruginosa 15442 and Klebsiella sp, within the first 30 s of exposure to the gel and within the first 60 s of exposure using the Escherichia coli 19538

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Summary

Introduction

Most of the infections present in hospitals and medical facilities are linked with poor hygiene practices at the moment of patience assistance, this is known to be a public health issue. The right sanitization of the health care personnel’s hands is considered an issue of vital importance to prevent and control infections [1]. It becomes necessary to use effective products to counter the propagation of infectious microorganisms and diminish the risk of contracting diseases, some of the products that are used in the medical facilities are antiseptic rubs, these leave-on products are highly recommended after a plain soap and water hand wash, because most of the time the soap does not have the antibacterial biocidal properties to destroy or to inhibit the development of the targeted microorganisms that can be present on living tissue [2, 3]. The technique used during the process was the dilution-neutralization technique [4, 5]

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