Abstract
Phenolics were extracted from the fruit tissues of Ananas comosus L. var. queen, cv. ‘Meitei Keehom’, a variety of pineapple grown in Manipur, India, after skin peeling, purified and their antioxidant properties were analyzed. The antioxidant properties were assessed based on the ability of fruit phenolics in absolute methanol to scavenge DPPH, superoxide anion radicals and hydroxyl radicals and compared to antioxidant compounds like ascorbic acid and pyragallol. Pineapple fruit phenolics scavenged DPPH, superioxide anion radicals and hydroxyl radicals in a dose dependent way. In DPPH assay, the IC50 values of pineapple phenolics, ascorbic acid and pyragallol were 12.2μg/ml, 17.82μg/ml and 15.92μg/ml respectively. In superoxide anion and hydroxyl radical scavenging activities, the IC50 values of pineapple phenolics were 11.42μg/ml and 55.292μg/ml, for ascorbic acid 49.62μg/ml, 48.52μg/ml and that of pyragallol was 15.672μg/ ml and 60.62μg/ml. The IC50 value was lowest in pineapple phenolics than ascorbic acid and pyragallol in DPPH and superoxide anion assays. But it is higher than ascorbic acid and lower than pyragallol in hydroxyl radical assay. The lower the IC50 values, the higher the antioxidant activities. The phenolics extracted from this variety of pineapple exhibit excellent free radical scavenging activity. The result shows that pineapple and its active constituents may be used in further antioxidative therapy.
Highlights
Ananas comosus L. belongs to Bromeliaceae family is a tropical to subtropical fruit native to Thailand, Phillipines, China, Brazil and India
The pineapple phenolics exhibited a dose dependent DPPH radical scavenging activity. These pineapple phenolics shows the highest scavenging activity followed by pyragallol and ascorbic acid respectively
The phenolics extracted from this variety of pineapple exhibited excellent antioxidant activity
Summary
Ananas comosus L. belongs to Bromeliaceae family is a tropical to subtropical fruit native to Thailand, Phillipines, China, Brazil and India. Pineapple fruit contains large amount of phenolics. Polyphenols possess outstanding antioxidant and free radical scavenging properties suggesting a possible protective role in man (Laughton, 1999; Scott et al, 1993). Their antioxidant potential is closely related to the number of hydroxyls, the higher the number, the more potent the chain breaking antioxidant action of the compound (Rafat Hussain et al, 1987). The antioxidant activity of phenolics is related to a number of different mechanisms such as free radical scavenging, hydrogen donation, singlet oxygen quenching, metal ion chelation and acting as a substrate for radical such as superoxide and hydroxide
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