Abstract
The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.
Highlights
Pistacia khinjuk Stoks is an evergreen shrub or tree of the family Anarcadiaceae
The antibacterial activity of Pistacia khinjuk Stoks essential oil was tested against eight bacterium species: Gram-positive Bacillus cereus ATCC 23456, Listeria monocytogenes ATCC 15313, Staphylococcus aureus subsp
The researchers introduced that the broth microdilution method was a rapid quantitative determining of minimal inhibitory concentration (MIC) based on measurement of the bioluminescent signal [6, 7]
Summary
Pistacia khinjuk Stoks is an evergreen shrub or tree of the family Anarcadiaceae. This specie is native to Turkey, Syria, Iraq, Iran, Egypt, Afghanistan and Pakhistan [1]. It has been used for a long time as useful remedies for different diseases, for example, the fruits of P. atlantica, P. khinjuk, and P. terebinthus for their aphrodisiac activity and treatment of liver, kidney, heart, and respiratory system disorders, and the gum resin of P. lentiscus, P. atlantica, P. khinjuk, and P. terebinthus for their wound healing activity, and treatment of brain and gastrointestinal disorders [2]. A lot of research has been undertaken on the properties of mastic gum. The same group of researchers [3] confirmed that mastic gum kills
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