Evaluation of acute and sub-acute toxicity of sivanar amirtham in albino mice and wistar albino rats

Abstract

The objective of present study is to evaluate the acute and sub-acute toxicity of Sivanar Amirtham in Swiss albino mice and Wistar albino rats. A single dose of extract (2000 mg/kg) was given orally to the animals in the acute study, and they were observed for 14 days. In the sub-acute investigation, biochemical, haematological, and histological parameters were assessed after successive doses of Sivanar Amirtham (100, 200, and 400 mg/kg/day) were given for 28 days. After a single or repeated dosage of Sivanar Amirtham, there was no evidence of toxicity or mortality. Sivanar Amirtham median lethal dose (LD50) was determined to be greater than 2000 mg/kg. When comparing the weights of the treatment and control groups, the Sivanar Amirtham caused no significant differences in body weights or organ weights. Most biochemical and haematological markers were within normal limits. Only the Sivanar Amirtham doses of 200 mg/kg and 400 mg/kg caused significant increases in MCV levels in both male and female rats. Significant increases in SGPT levels were seen in both male and female rats at dosages of 200 mg/kg and also increase in the activities of SGPT in female rats at a dosage of 400 mg/kg Sivanar Amirtham (p<0.05 and p<0.01, respectively). Every organ's histological evaluations were normal, except a tiny change in the liver and kidney. Oral administration of the Sivanar Amirtham is considered relatively non-toxic. Keywords: Sivanar Amirtham, Acute Toxicity, Sub-acute Toxicity, Hematology, Serum biochemistry, Histopathology.