Abstract
Several biodegradation flask systems were evaluated to assess the effects of flask shape and volume and length of incubation on the recovery of 14CO2. During a 6-h incubation period, it was demonstrated that the recovery of 14 CO2 decreased as the volume of the flask (50 ml to 2 L) and the length of the diffusion path increased. Increasing the incubation time improved the performance of flasks, which were relatively inefficient at 6 h. Selected flask systems were further assessed, measuring the extent of CO2 evolution from nonradiolabeled linear alkylate sulfonate and glucose by a mixed inoculum.
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