Abstract
A set of 42 SSRs of wheat were evaluated for their cross-amplification on the DNA ofThinopyrum ponticum, Thinopyrum intermedium, Thinopyrum elongatum, Thinopyrum bessarabicum, Pseudoroegneria stipifolia,andDasypyrum villosum.The number of the wheat SSR markers that amplified DNA fragments with determined size forTh. ponticumwas 33 (78.6%); forTh. intermedium,28 (66.7%); forTh. elongatum,24 (57.1%); forTh. bessarabicum,24 (57.1%); forP. stipifolia,26 (69.1%); and forD. villosum,29 (69.0%). Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The dataset can be used for phylogenetic studies of wild relatives of wheat, for the estimation of their diversity, and for the introgression of agronomically valuable genes into wheat genome.
Highlights
Wheat is a major food source for most world population
In order to manage sustainable wheat production, it is necessary to develop new cultivars of wheat resistant and tolerant to adverse environmental factors. e genetic diversity of wheat is restricted due to the bottleneck effect which occurred during domestication and, on the other hand, due to the limited diversity of wheat germplasm used in the breeding programs [1, 2]. e genetic variability of cultivated wheat can be improved by wide hybridization involving wild relative species
The development of new Simple Sequence Repeats (SSRs) markers de novo for wild relatives of wheat is a time- and costconsuming process. e anking regions of microsatellites show sufficient homology between closely related species. us, the primers of wheat SSR markers can be used in the analysis of other Poaceae species. is is called “transferability” and it has been successfully used for cultivated [8] and wild (Elymus, Aegilops) species [9, 10]. e goal of our study was to test a set of 42 microsatellite markers of wheat for their ability to cross-amplify DNA from the accessions of . intermedium, . elongatum, . ponticum, . bessarabicum, P. stipifolia, and D. villosum
Summary
Wheat is a major food source for most world population. its cultivation is strictly limited by such challenges as pests, diseases, droughts, extreme temperatures, and others. E genetic variability of cultivated wheat can be improved by wide hybridization involving wild relative species. Ese genetic resources can be efficiently used in wheat breeding by application of molecular microsatellite markers. Ey have been successfully used as DNA markers to estimate genetic diversity, genome mapping, and marker-assisted selection for agronomically important traits [5]. SSRs can be used in wide hybridization of wheat to monitor and map desirable alien genes in segregating populations as they are locus speci c and inherited in codominant manner [7]. The development of new SSR markers de novo for wild relatives of wheat is a time- and costconsuming process. Us, the primers of wheat SSR markers can be used in the analysis of other Poaceae species. The development of new SSR markers de novo for wild relatives of wheat is a time- and costconsuming process. e anking regions of microsatellites show sufficient homology between closely related species. us, the primers of wheat SSR markers can be used in the analysis of other Poaceae species. is is called “transferability” and it has been successfully used for cultivated (barley, oats, and rye) [8] and wild (Elymus, Aegilops) species [9, 10]. e goal of our study was to test a set of 42 microsatellite markers of wheat for their ability to cross-amplify DNA from the accessions of . intermedium, . elongatum, . ponticum, . bessarabicum, P. stipifolia, and D. villosum
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