Evaluating the Trapping Potential of Rosa damascena and Phenylacetaldehyde for Paranthrene diaphana

Essential oils and isolated essential oil compounds are known to exert various pharmacological effects, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-immunomodulatory, antioxidant, and wound healing effects. Based on selected articles, this review deals with the potential antiviral and virucidal activities of essential oils and essential oil compounds together with their mechanism of action as well as in silico studies involving viral and host cell-specific target molecules that are indispensable for virus cell adsorption, penetration, and replication. The reported in vitro and in vivo studies highlight the baseline data about the latest findings of essential oils and essential oil compounds antiviral and virucidal effects on enveloped and non-enveloped viruses, taking into account available biochemical and molecular biological tests. The results of many in vitro studies revealed that several essential oils and essential oil compounds from different medicinal and aromatic plants are potent antiviral and virucidal agents that inhibit viral progeny by blocking different steps of the viral infection/replication cycle of DNA and RNA viruses in various host cell lines. Studies in mice infected with viruses causing respiratory diseases showed that different essential oils and essential oil compounds were able to prolong the life of infected animals, reduce virus titers in brain and lung tissues, and significantly inhibit the synthesis of proinflammatory cytokines and chemokines. In addition, some in vitro studies on hydrophilic nano-delivery systems encapsulating essential oils/essential oil compounds exhibited a promising way to improve the chemical stability and enhance the water solubility, bioavailabilty, and antiviral efficacy of essential oils and essential oil compounds.

Abstract. Hartatie ES, Prihartini I, Widodo W, Wahyudi A. 2020. Short Communication: Detection of bioactive compounds in essential oil from lemongrass cultivated in Ngantang, Malang, East Java, Indonesia. Biodiversitas 21: 2821-2825. Lemongrass (Cymbopogon citratus) commonly known as West Indian lemongrass is a member of the Poaceae family and belongs to genus Cymbopogon. It is one of the plants commonly used in fresh form as a spice by the people in Indonesia and widely planted in the yard of the house. Lemongrass contains several bioactive compounds and it might be extracted to obtain essential oils useful for many applications such as flavor and fragrance ingredients, perfumery, cosmetics, food preservation, pharmaceutical. The chemical composition of essentials oil can be variable, according to the genetic diversity, habitat, weather, and cultural treatment. The aim of study was to investigate the bioactive compounds in essential oil from lemongrass stalk and lemongrass leaves which cultivated in Ngantang, Malang, East Java, Indonesia. Detection of bioactive compounds was carried out by gas chromatography-mass spectrometry (GC-MS). The results of study are the main compound in lemongrass essential oil was citral. There is a difference between the citral content of essential oils from lemongrass stalk compared to essential oils from lemongrass leaves, both in quantity and components. The citral (geranial and neral) content of essential oil made from stalk was higher than made from leaves, but the amount of bioactive compounds of essential oils from leaves more than from the stalk.

The purpose of this study was to assess the effectiveness of essential plant oils from Vitex agnus-castus L. (VAC) and Myrtus communis L. against the plant pathogens, Fusarium oxysporum f. sp. radicis-lycopersici (Sacc.) W.C. Synder & H.N. Hans, Rhizoctonia solani J.G. Kühn., Sclerotinia sclerotiorum (Lib.) de Bary and Verticillium dahliae Kleb., and to determine the chemical composition of the compounds in these essential oils. GC/MS analysis was identified 25 different compounds in VAC essential oil, while the main compounds were determined as Eucalyptol (17.75%), β-Caryophyllene (13.21%) and Spathulenol (10.41%). On the other hand, the essential oil of M. communis, consisted of 16 different compounds which were Eucalyptol (49.15%), Myrtenol (19.49%) and α-Pinene (8.38%) being its main compounds. An assessment of antifungal activity was performed under in vitro conditions. Plant pathogens were inoculated onto Petri dishes (60 mm) containing PDA medium (10 mL/Petri-1), and plant essential oils were applied at concentrations of 0.5, 1, 1.5, 2, 5 and 10 (μL/Petri-1) into the 5 mm diameter wells opened on the Petri dish surface. After that, the Petri dishes incubated at 22±2 °C. The results of this study, the essential oil of M. communis, at a dose of 10 μL/ Petri, inhibited the 100% mycelium growth of V. dahliae, S. sclerotiorum and R. solani. The highest dose of VAC essential oil was also 100% inhibited V. dahliae and S. sclerotiorum. The LC50 and LC90 values of M. communis and VAC essential oil calculated for V. dahliae, FORL, S. sclerotiorum and R. solani. This plant extracts were shown by in vitro conditions to be potential antifungal agents.

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.

Maternal food cues during pregnancy and lactation have been demonstrated in several mammalian species, including pigs, to impact offspring food preferences later in life (Hepper et al. 2012). However, the evidence that dietary volatile compounds are transferred, and to what extent, into maternal fluids in pigs remains elusive. We hypothesise that the efficiency of transfer of dietary compounds into maternal fluids will be specific to each compound and related to their chemical nature. This study aimed to trace and quantify dietary essential oil (EO) compounds in milk and amniotic fluid in sows. A total of 38 multiparous Large White sows were selected at 104 days gestation. The experiment was divided in two trials: Trial 1 (T1) aimed to assess the kinetics of the potential transfer of two EO compounds (geraniol and anethole) as a proof-of-concept; and Trial 2 (T2) studied the transfer of eight different EO (oregano, thyme, clove, cinnamon, lemon myrtle, lemon ironbark, peppermint gum and nerolina). In T1, six sows per treatment were fed a normal gestation or lactation diet supplemented with one morning dose consisting of 450 ppm of each EO (EO1.1), or the same total amount of each EO but administered in two meals with 225 ppm of each compound (EO1.2). Control group (C1) received non-supplemented feed. Amniotic fluid samples were collected by squeezing the placental tissue. Milk/colostrum was collected on 1 d and 5 d lactation hourly for 6 h immediately following the morning meal. In T2, eight EO were added in equal amounts to feed to a final dose 1 kg/ton (EO2) and compared to a non-supplemented control group (C2). Sows were induced to farrow in order to be able to collect fresh amniotic fluid samples. Colostrum was collected on d 1 lactation, 1 h after the morning meal. All samples were stored at −20°C and analysed by GC-MS. The statistical analysis included t-test and ANOVA (Minitab 16, Minitab Inc., State College, PA, USA). Results for the T1 showed a significant increase of geraniol and anethole in colostrum (P = 0.02 and P = 0.036 respectively). However, no significant differences (P > 0.5) could be measured in amniotic fluid. Results for T2 are shown in Table 1. All dietary EO were significantly transferred to amniotic fluid and colostrum, except for lemon myrtle. The results also showed significant differences (P = 0.001) in the rate of transfer of the different EO, thyme being the most efficiently transferred to colostrum and clove the most efficient in amniotic fluid. Overall, there was a higher (P < 0.05) transference to amniotic fluid than colostrum for all the EO except peppermint gum, cinnamon and oregano. Table 1. Trial 2 results on transfer of dietary EO to amniotic fluid and colostrum in sows comparing the control (C) and the EO treated (EO) groups (See attachment) In conclusion, our data proves that all dietary EO tested, except lemon myrtle, were transferred to maternal fluids in sows, but in a different rate and quantity. The results confirmed that dietary volatile compounds might be present in colostrum and amniotic fluid of sows and therefore foetuses and newborn piglets could potentially experience perinatal conditioning, hence improving weaning, welfare and performance.

Objective/Background: This work aims to evaluate the chemical composition of Paramignya scandens essential oils (EOs), their in vitro α-amylase, tyrosinase inhibitory activities, and in silico molecular docking study. Methods: The EOs of P scandens trunks and leaves were extracted by hydrodistillation process and the chemical compositions were determined by gas chromatography-mass spectrometry. These EOs were also tested for in vitro α-amylase and tyrosinase inhibitory activities. Additionally, in silico molecular docking simulations on the main components of the EOs were performed. Results: The EO profiles of P scandens trunks and leaves included 21 (98.8%) and 34 (89.7%) components, respectively. The main compounds in the trunk EO were ( E)- β-caryophyllene (60.8%), α-humulene (13.0%), and germacrene D (7.4%). The predominant compounds of the leaf EO were caryophyllene oxide (16.7%), ( E) -β-caryophyllene (14.6%), spathulenol (12.2%), germacrene D (6.5%), and α-humulene (5.0%). Moreover, their α-amylase and tyrosinase inhibitory effects were demonstrated for the first time. In molecular docking results, ( E)- β-caryophyllene was the potential compound in EOs with the best binding affinity against the α-amylase enzyme, whereas spathulenol revealed the strongest binding ability with the tyrosinase enzyme. Furthermore, the assessment of potential interactions between the main components of the EOs and the selected proteins was elucidated. Conclusions: The results of this work revealed that EOs of P scandens trunks and leaves had α-amylase and tyrosinase inhibition properties and potential applications in nutraceuticals, medicine, and cosmetics.

Lavandula sp. essential oil and hydrolate are commercially valuable in various industry branches with the potential for wide-ranging applications. This study aimed to evaluate the quality of these products obtained from L. x intermedia cv. ‘Budrovka’ for the first time cultivated on Fruška Gora Mt. (Serbia) during three successive seasons (2019, 2020, and 2021). Essential oil extraction was obtained by steam distillation, and the composition and influence of weather conditions were also assessed, using flowering tops. The obtained essential oils and hydrolates were analysed by gas chromatography with a flame ionization detector (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). A linear regression model was developed to predict L. x intermedia cv. ‘Budrovka’ essential oil volatile compound content and hydrolate composition during three years, according to temperature and precipitation data, and the appropriate regression coefficients were calculated, while the correlation analysis was employed to analyse the correlations in hydrolate and essential oil compounds. To completely describe the structure of the research data that would present a better insight into the similarities and differences among the diverse L. x intermedia cv. ‘Budrovka’ samples, the PCA was used. The most dominant in L. intermedia cv. ‘Budrovka’ essential oil and hydrolate were oxygenated monoterpenes: linalool, 1,8-cineole, borneol, linalyl acetate, and terpinene-4-ol. It is established that the temperature was positively correlated with all essential oil and hydrolate compounds. The precipitations were positively correlated with the main compounds (linalool, 1,8-cineole, and borneol), while the other compounds’ content negatively correlated to precipitation. The results indicated that Fruška Gora Mt. has suitable agro-ecological requirements for cultivating Lavandula sp. and providing satisfactory essential oil and hydrolate.

A high-performance liquid chromatography (HPLC) method was established using an analytical reversed-phase column and gradient elution to achieve chromatographic separation of typical compounds in essential oils. For detection, a diode array detector monitoring different wavelengths simultaneously as well as a mass spectrometer (MS) were used. Atmospheric pressure chemical ionization operating in the positive mode turned out to be a suitable tool to detect volatiles of different chemical classes and to identify them in essential oil matrices. Characteristic fingerprints of eucalyptus, lavender, may chang, pine, rosemary, thyme, and turpentine essential oils monitored at a representative wavelength (220 nm) demonstrated the suitability of HPLC in essential oil analysis. Additional monitoring wavelengths (210, 250, and 280 nm) provided useful information about the identity of the specific component and opened the possibility to differentiate presumably coeluting compounds by means of their distinct absorption behavior. Finally, peak assignment in seven essential oils was performed on the basis of characteristic retention times and UV and MS data of a broad set of reference volatiles.

Damask rose (Rosa damascena Mill.) is a high-value aromatic plant species belonging to the family Rosaceae. It is being cultivated throughout the world for rose essential oil production. Besides its higher demand in the aromatic and cosmetic industry, the essential oil obtained has many pharmacological and cytotoxic activities. The primary concern of growers with the available varieties of damask rose is short flowering duration, low essential oil content and unstable yield. Thus, there is a requirement for developing new stable varieties with higher flower yield and essential oil content. The present study evaluated the variations in the flower yield parameters, essential oil content, and essential oil compounds in different clonal selections of damask rose. These clonal selections have been developed through a half-sib progeny approach from commercially available varieties 'Jwala' and 'Himroz.' The fresh flower yield varied from 629.57 to 965.7 g per plant, while the essential oil content ranged from 0.030–0.045% among the clonal selections. The essential oil profiling via gas chromatography–mass spectrometry revealed significant variations in the essential oil compounds. Acyclic monoterpene alcohols citronellol (20.35–44.75%) and geraniol (15.63–27.76%) were highest, followed by long-chain hydrocarbons, i.e., nonadecane (13.02–28.78%). The clonal selection CSIR-IHBT-RD-04 was unique in terms of the highest citronellol content (44.75%) and citronellol/geraniol (C/G) ratio of 1.93%. This selection has the potential use as a parental line in future genetic improvement programs of damask rose to achieve higher yield and better quality of rose essential oil.

Meta-analysis of the effects of essential oils on ruminal fermentation and performance of sheep

Context: Essential oils are secondary metabolites with versatile organic structures that, due to their compounds, have useful medicinal properties. There are about 250 species of the genus of Scutellaria perennial flowering plants from the Lamiaceae family. Its application for the treatment of allergy, inflammatory, hyperlipidemia, arteriosclerosis, hypertension, and hepatitis has a long history. Evidence Acquisition: Various studies on the chemical compounds of the Scutellaria genus have identified several ‎compounds, especially essentials oils. The current review is based on the evidence found in Chemical Abstract, Science Direct, Scopus, PubMed, Web of Knowledge, and Google Scholar databases. Results: Many studies on the chemical components of essential oils from the Scutellaria genus have identified several compounds. We summarized the chemical compositions and biological activities of Scutellaria essential oils. Hexadecanoic acid, germacrene D, β-caryophyllene, linalool, β-farnesene, and eugenol are the main compounds in essential oils of this genus. Despite many reports about essential oils of Scutellaria species (more than 38), a large number of species have not been studied yet. Therefore, several studies should be conducted on the chemical compounds and biological activities of unstudied Scutellaria essential oils. Conclusions: This review has summarized reports on the chemistry and biological activities of Scutellaria essential oils,‎ such as antioxidant, antimicrobial, antifeedant, phytotoxic, and acaricidal toxicities, based on the recent literature.

The essential oils from the Centaurea genus are well known for their pharmacological properties. The most abundant and dominant chemical components in Centaurea essential oils are ß-caryophyllene, hexadecanoic acid, spathulenol, pentacosane, caryophyllene oxide, and phytol. However, whether these dominant components are the key drivers for observed antimicrobial activity remains unclear. Thus, the aim of this study was dual. Here we provide comprehensive, literature-based data to correlate the chemical compounds in Centaurea essential oils with the tested antimicrobial activity. Secondly, we characterized the essential oil of Centaurea triumfettii All. squarrose knapweed using coupled system gas chromatography–mass spectrometry and tested its phytochemicals for antimicrobial activity against E. coli and S. epidermis using disc diffusion assay and monitoring their growth in Muller Hinton broth. The most abundant compounds in C. triumfettii essential oil were hexadecanoic acid (11.1%), spathulenol (10.8%), longifolene (8.8%), germacrene D (8.4%), aromadendrene oxide (6.0%) and linoleic acid (5.3%). Based on our analysis of literature data from other Centaurea essential oils, they were positively correlated with antimicrobial activity. Using an agar disk diffusion method, tested chemical constituents did not show experimental evidence to support this positive correlation to antimicrobial activity when we tested them as pure components. The antibacterial effect of essential oil constituents may be related to a complex synergistic, rather than a single component as suggested by performed network pharmacology analysis, underlying the theoretical interactions between the essential oil phytochemicals listed as potentially responsible for antimicrobial activity and should be confirmed in further in-depth studies. This is the first report on the comparative analysis of Centaurea essential oils with good antimicrobial activity, as well as the first analysis of chemical components of the essential oil from C. triumfettii and the first report of antimicrobial activity of the representative, pure components: aromadendrene, germacrene D, spathulenol, longifolene, and the mixture of selected chemical compounds. This work contributes to the body of knowledge on the genus Centaurea and C. triumfettii species.

Polygonum minus Huds. is an aromatic plant with potential anti-inflammatory, antiulcer and diuretic properties. Although the volatile compounds from the essential oil of P. minus have been extensively studied, knowledge of the aroma-active compounds is still incomplete. Hence, the aim of this study is to investigate and to characterise the aroma-active compounds that contribute to the potent odour of P. minus. Essential oil was extracted from the leaves of kesum (P. minus) using hydrodistillation and was analysed by gas chromatography-mass spectrometry (GC-MS) to identify the volatile constituents. The important aroma-active compounds were further analysed by GC-MS/Olfactometry (GC-MS/O) and aroma extraction dilution analysis (AEDA). The GC-MS analysis using Kovats retention indices detected 39 volatile compounds in the essential oil. Aldehydes represented the major class of compounds, encompassing 75.43% of the total peak area, followed by sesquiterpenes (13.19%), alcohols (8.07%) and organic acids (0.83%). The dominant components of the kesum essential oil were dodecanal (54.25%) and decanal (18.27%) in the aldehyde group. The sesquiterpenes that were detected include β-farnesene (1.74%), α-caryophyllene (1.72%), β-caryophyllene oxide (1.51%) and βselinene (1.36%). In this study, GC-MS/O and AEDA analysis identified dodecanal, decanal, farnesol, 1-nonanal and α-bergamotene as the main contributors to the characteristic fragrance of this plant. This is the first olfactometric study on the essential oil of P. minus, and it is important for our understanding of the biosynthesis of volatile compounds in this medicinal herb. Moreover, the characterisation of aromatic compounds that contributed to the potent odour of P. minus can be further applied in the flavour and fragrance industries.

Limau kuit is believed to be a local orange from South Kalimantan. Limau kuit has a distinctive aroma different from other oranges and is thought from its essential oil. The essential oil from limau kuit has the potential to be extracted and produced into valuable goods. Limau kuit essential oil from a combination of leaves and twigs has never been reported before. The composition of essential oils is influenced by genetic and environmental factors. The region producing limau kuit in Kabupaten Banjar is in Kecamatan Astambul and Kecamatan Aranio. These places geographically have different conditions, especially the altitude of the location, so it is thought to have a difference in the essential oils. This research aims to obtain essential oil from the leaves and twigs of limau kuit by hydrosteam distillation and determine the characteristics and composition of each plant's origin. Hydrosteam distillation was carried out for 6 hours at 60-70 °C. The essential oil of leaves and twigs of limau kuit is transparent pale yellow and has an aroma similar to the source with an average yield of 0.6027% (v/w) or 0.5062% (w/w). The specific gravity, refractive index, optical rotation, and solubility in alcohol, respectively, of Astambul essential oil are 0.8540; 1.4792; (-14.05); and 1:6; and Aranio 0.8495; 1.4792; (+)0.16, and 1:7. Based on the results of GC-MS analysis, the components of Astambul and Aranio essential oils overall tend to be the same with a difference of 25%. Number of compounds in essential oil from Astambul was 32, while from Aranio was 38. The three main compounds in essential oils from Astambul and Aranio are g-terpinene, o-cymene, and (+)-bicyclogermacrene, with the respective %area in Astambul essential oil, was 25%; 13.22%, and 6.38%; and Aranio was 22.43%; 11.68%; and 5.88%.KEYWORD : Limau kuit leaves-twigs; essential oil; hydrosteam distillation; Astambul; Aranio.

Citrus aurantiifolia (Christm) Swingle: Biological potential and safety profile of essential oils from leaves and fruit peels