Evaluating the thrombolytic property of Juniperus Sabina tincture using spectrophotometry

Abstract

Objectives: We aimed to explore the thrombolytic property of Juniperus Sabina by measuring the absorbance level of released haemoglobin (Hb) during clot lysis. Materials and Methods: Approximately 0.3 mL of blood was added to four 15 mL centrifuge tubes and blood clots were allowed to form naturally. The clots were incubated for 45 min and washed with normal saline. Using a visible spectrophotometer, the absorbance of Hb was recorded at five wavelengths: 405, 416, 541, 576 and 580 nm. Lyophilised recombinant streptokinase (SK) was used as standard control. J. Sabina mother tincture (83.28% v/v) of 1:10 (T1) and 1:20 (T2) dilutions were studied. Distilled water and ethyl alcohol mixed in a ratio of 1:1 (hydro alcohol) were used as a negative control. Results: Thrombolytic activity was compared by recording the absorbance of Hb at five wavelengths (405, 416, 541, 576 and 580 nm) every 10 min from 0 to 60 min. Absorption of light by the released Hb during thrombolysis was highest at 416 nm for SK, T1 at 405 and 416 nm and HA and T2 at 405 nm. Comparatively, T1 showed higher absorption than HA. In general, the peak absorbance of Sabina concentrations was more when compared to negative controls in the higher wavelengths of 541, 576 and 580 nm. However, this difference was not statistically significant. The mean and standard deviation from the data were calculated to find the amount of dispersion for all the samples at different wavelengths. The within- and between-group significance was determined using analysis of variance. Conclusion: This study has shown that Sabina in the dilutions of 1:10 and 1:20 does not induce thrombolysis in vitro. By and large, further studies with different concentrations of Sabina Q with a sophisticated spectrophotometer need to be tested for thrombolytic activity.