Abstract
The study aims to explore microbial lipid production using an abundant and low-cost lignocellulosic biomass derived from forestry residues. Sugar-rich undetoxified hydrolysate was prepared using hardwood and softwood sawdust and used for lipid production as a carbon source from an oleaginous yeast, Rhodosporidium toruloides-1588. The maximum biomass obtained was 17.09 and 19.56 g/L in hardwood and softwood hydrolysate, respectively. Sugar consumption in both hydrolysates was >95%, with a maximum lipid accumulation of 36.68% at 104 h and 35.24% at 96 h. Moreover, R. toruloides-1588 exhibited tolerance to several toxic compounds such as phenols, organic acids and furans present in hydrolysates. The lipid characterization showed several monosaturated and polyunsaturated fatty acids, making it a potential feedstock for biofuels and oleochemicals production. This study confirms the credibility of R. toruloides-1588 as a suitable lipid producer using hydrolysates from forestry residues as a substrate. Additionally, lipids obtained from R. toruloides-1588 could be a potential feedstock for advanced biofuel production as well as for food and pharmaceutical applications.
Highlights
A microorganism tends to accumulate lipids in the form of triglycerides, under the nutrient stress conditions, either during nitrogen limitation or excess of carbon [1]
The hydrolysate was prepared from sawdust residues of the Maple tree and Fir tree
Forestry biomass as a raw material to obtain liquid hydrolysates for the production of biofuel feedstock was investigated in the present study
Summary
A microorganism tends to accumulate lipids in the form of triglycerides, under the nutrient stress conditions, either during nitrogen limitation or excess of carbon [1]. Microorganisms that can store more than 25% lipid (w/w) of their total dry cell weight (DCW) are known as oleaginous strains. Lipomyces starkeyi, Yarrowia lipolytica, Rhodosporidium toruloides, Cryptococcus curvatus, among others. Their capability to thrive on a wide variety of inexpensive substrates renders them an outstanding alternative to conventional oil sources [2]. Microbial lipids have applications in several industries including biofuels, food, pharmaceutical, and chemical industries. The critical challenges in lipid production are the availability of substrates, process cost, and that they should comply with food security. Biorefinery has moved towards the usage of lignocellulosic-based biomass as an alternative substrate for microbial-based lipid production [2]
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