STAR protocols | VOL. 3

Evaluating the glycolytic potential of mouse costimulated effector CD8+ Tcells ex vivo.

Publication Date Jun 17, 2022


Studying the metabolic fitness of Tcells is fundamental to understand how immune responses are regulated. Here, we describe a step-by-step protocol optimized to efficiently generate and isolate effector antigen-specific CD8+ Tcells ex vivo using costimulation. We also detail steps to evaluate their metabolic activity using Seahorse technology. This protocol can be used to measure the glycolytic potential of effector murine Tcells in response to different manipulations, such as infections, adjuvant studies, gene editing, or metabolite supplementation. For complete details on the use and execution of this protocol, please refer to Agliano etal. (2022).


Seahorse Technology Gene Editing Metabolic Activity Immune Responses Glycolytic Potential Metabolic Fitness Murine Tcells Antigen-specific CD8 Effector CD8

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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