Evaluating Expression of Gene Encoding Flavonol Synthase in Green TrungDu and Purple TrungDu by Real time PCR and HPLC Technique

Abstract

In plants, flavonol synthase (FLS) is a multifunctional enzyme that converts dihydroflavonol into flavonols and naringenin into dihydrokaempferol. FLS from tea has been shown to metabolize dihydroquercetin to quercetin. In this study, we conducted studying on the relationship between quercetin content and the expression level of FLS in two traditional tea cultivars of Vietnam, TrungDuxanh and TrungDutim tea grown in tea garden of Thai Nguyen University of Agriculture and Forestry. Tea shoots with one apical bud and two to three young leaves using as research materials which collected in September 2017. The using of HPLC technique did not detect the quercetin content from the these tea samples. The preliminary results of quantification of FLS gene expression by real time PCR showed the expression level of FLS in green Trung Du tea is higher than purple Trung Du, although the difference is not great. Thus, at the time of collecting, the expression of FLS in green Trung Du and purple Trung Du can not synthesize quercetin but synthesize other flavonols.