Evaluating bridge‐building strategies: How direct and indirect contact with refugees in Italian welcome projects promotes positive intergroup relations

We observed that in vitro transcriptomic response of bovine oviduct epithelial cells (BOEC) to the early embryo could be the result of a contact-dependent signalling effect or interactions with embryo secretions. In order to determine this, BOEC were co-cultured directly with embryos or indirectly with embryo-conditioned media (CM); BOEC from the isthmus of oviducts at early luteal phase were cultured with TCM-199+10% fetal calf serum (FCS) in 4-well plates in 5% CO2 in air at 38.5°C for 6 days until confluence. In vitro 2- and 8-cell embryos as well as their CM were produced in parallel. A day before co-culture, BOEC medium was replaced with SOF+10% FCS. Groups for 2- and 8-cell embryos were established: BOEC in direct contact with embryos; BOEC in the same well as embryos but not in indirect contact; BOEC with embryo CM; and BOEC without embryos, as a control. Polyester mesh was used to maintain embryos position on top of the cells. After 48 h of co-culture, BOEC were recovered for gene expression analysis (4 replicates). The relative abundance of candidate genes previously shown to be affected by the presence of embryo in vivo (Maillo et al. 2015 Biol Reprod. 92, 144) [SMAD6 (BMP signalling pathway); ROCK1, ROCK2 (cytokinesis); SOCS3 (inflammatory response); PRELP (extracellular matrix)] or in vitro (Schmaltz-Panneau et al. 2014 Anim. Reprod. Sci. 149, 103-106) [GPX4, NFE2L2 (oxidative stress); SCN9A (sodium ion binding); EPSTI1 (tissue remodelling); IGFBP3 (insulin-like growth factor binding); TDGF1 (BMP signalling pathway); AGR3 (regulation of ciliary beating)] was assessed by RT-qPCR. H2A.Z and ACTG1 were used as housekeeping genes. Statistical analysis was assessed by ANOVA. The BOEC responded to the presence of 2-cell embryos only when in direct contact by significantly decreasing abundance of NFE2L2. Both direct and indirect embryo contact or culture with CM significantly decreased GPX4, ROCK2, and SCN9A transcripts compared with control. The presence of 2-cell embryos irrespective of being in direct or indirect contact reduced the expression of SMAD6 compared with the control and CM groups. In the case of CM, expression of IGFBP3 was enhanced compared with the control but was similar to the presence of the 2-cell embryos. In the presence of 8-cell embryos, direct contact with BOEC significantly down-regulated the expression for GPX4 and SOCS3, whereas expression of SCN9A was up-regulated. The opposite was observed when compared with control. The presence of 8-cell embryos down-regulated the expression of SMAD6 and ROCK2 compared with the CM group, whereas direct or indirect contact with BOEC or culture with CM down-regulated the expression of PRELP compared to control. In conclusion, these results provide evidence for a differential affect on the transcriptome of BOEC in vitro depending on embryo stage. These changes may be related either with direct embryo contact or embryo secretions released into the media. Research supported by Spanish MINECO-AGL2015-70140-R; AGL2015-66145-R; OECD-Co-operative Programme TAD/CRP JA00092482.

Contact with mental health consumers has shown to be a promising strategy to address mental health stigma, particularly in the context of pharmacy education. This research aimed to compare the effectiveness of a direct (face-to-face) contact intervention with an indirect (film based) contact intervention in reducing the mental health stigma of pharmacy students. A two-group, non-randomized, comparative study was conducted with third year pharmacy students (n = 198) allocated to the direct contact arm and fourth year pharmacy students (n = 278) allocated to the indirect contact arm. Baseline and immediate post-intervention data were collected using a validated 39 item survey instrument to assess the impact of the interventions on mental health stigma as well as attitudes towards providing mental health pharmaceutical services. Participants in the direct contact group showed a significant improvement in 37 out of 39 survey items and participants in the indirect contact group showed a significant improvement in 27 out of 39 items (P < 0.05). While direct contact had a stronger impact than indirect contact for 22 items (P < 0.05), for numerous key measures of mental health stigma the impact of the two contact interventions was equivalent. Both indirect and direct contact may positively impact mental health stigma. While the strength of the stigma-change process may be heightened by face-to-face interactions, the largely positive impact of indirect contact suggests that stigma reduction may depend less on the medium of contact but more on the transcendent messages contributed by the consumers facilitating the contact experience.

Simulation of between-farm transmission of porcine reproductive and respiratory syndrome virus in Ontario, Canada using the North American Animal Disease Spread Model

This contribution examines the role of vicarious contact (observing in-group members having successful cross-group contact) as a tool to improve intergroup relations. Expanding previous research on indirect intergroup contact, vicarious contact (1) integrates and applies concepts of social-cognitive theory ( Bandura, 1986 ) to the field of intergroup contact research; (2) broadens the study of indirect contact effects to the observation of successful cross-group interactions; and (3) proposes to increase people’s intention for direct cross-group contact. Two video-based experiments indicate that vicarious contact improves attitudes towards the out-group and increases participants’ willingness to engage in direct cross-group contact. These studies provide evidence that the relation between vicarious contact and intergroup attitudes (and willingness to engage in direct contact) is sequentially mediated by self-efficacy expectancy and perceived intergroup uncertainty. Implications of these findings for further research on the (indirect) contact hypothesis and their application will be discussed.

Direct and indirect intergroup contact effects on prejudice: A normative interpretation

PurposeThe transgender community faces prejudice and stigma and is one of the most ostracised groups in society. One of the ways to reduce prejudice is through intergroup contact. This may be achieved through direct or indirect contact. The purpose of this paper is to compare the impact of direct and indirect contact on reducing transphobia.Design/methodology/approachDirect contact was achieved through a transgender speaker panel and indirect contact involved a video presentation. In total, 159 students enroled in undergraduate courses at a prominent university in India were enlisted for this study. Perceptions regarding transgenders were measured using the genderism and transphobia scale. Perceptions were measured at three different time points – before the contact, immediately after the contact and one month post contact.FindingsResults indicate that both direct and indirect contact cause a significant immediate decrease in transphobia at the post intervention stage. However, only direct contact caused significant reduction at the follow-up stage (one month after the intervention). Direct contact also effected a greater reduction in transphobia than indirect contact.Research limitations/implicationsThis study extends previous research that shows that speaker panels involving sexual minority speakers can result in reducing stigma (e.g. Croteau and Kusek, 1992). The present study shows that such speaker panels can also be useful for reducing stigma against transgender individuals. Another important outcome of this study is the relative effectiveness of direct contact in reducing transphobia compared to indirect contact. Direct contact resulted in greater reduction in transphobia both at the post-test and follow-up stages compared to indirect contact.Practical implicationsThe results of this study may benefit HR practitioners and policy makers in designing workplace initiatives and policies in creating an inclusive workplace. This study shows that meaningful interaction with transgenders would be a key step in reducing stigmatisation. Since direct contact is rarely expensive or time consuming, it can be a valuable tool to improve the integration of transgender individuals within society. Therefore, students and employees may be encouraged to interact with transgender individuals through panel discussions and workshops. Indirect contact may be used as a preliminary intervention in certain cases where direct contact may be difficult to organise.Social implicationsThe stigma faced by transgender individuals has a significant negative impact on their quality of life (Grant et al., 2014; Reisner and Juntunen, 2015). It is, therefore, necessary to recognise and reduce prejudice against transgenders at both the college and school levels as well as in work organisations. Educators and managers have a significant role to play in this societal change. This study shows that stigma reduction can be achieved in a fairly simple way through contact theory.Originality/valueThis study is one of the first to investigate Indian students’ perceptions of transgenders. It improves on earlier studies using similar interventions in two main ways. First, this study includes a follow-up assessment, which was not performed in most studies. Second, random assignment of participants to one of two conditions improves the reliability of the findings.

Allport's (1954) contact hypothesis predicted that pleasant contact with a member of a negatively stigmatized group would change attitudes both toward the specific person interacted with (specific attitude change) and also toward the group as a whole (generalization). Many previous studies of the contact hypothesis have demonstrated specific attitude change. In previous studies that demonstrated generalization, attitude change toward the group as a whole might have occurred because participants changed their opinions about what constituted a "typical" group member. Although this postulated mechanism of attitude change is difficult to test directly, our study sought indirect support by showing that preexisting conceptions of the typical group member differ in a way that affects the extent of generalization. Students who initially conceived of the typical person with AIDS (PWA) as an abstraction displayed greater generalization following pleasant contact with a PWA than did students who initially conceived of the typical PWA as a specific person. The generalization part of Allport's contact hypothesis may thus be related to recent research on social categorization.

According to the extended contact hypothesis, direct intergroup contact is not necessary for prejudice reduction; it suffices to know that ingroup friends have outgroup friends. However, extended contact is typically measured in a way that does not clarify whether people know the outgroup friend of their ingroup friend or whether they are even direct friends. A social network approach is used to compare extended contact when ingroup friends’ outgroup friends are not direct friends (open triads) to when there is a direct friendship with some (mixed triads) or all of the ingroup friends’ outgroup friends (closed triads). Results from a nonprobability sample in the US predicting feelings toward Black people ( N = 313) and from a representative sample in the Netherlands predicting attitudes toward immigrants ( N = 818) show that extended contact reduces prejudice only when some of the outgroup friends of one’s ingroup friends are known. This suggests that the extended contact effect should not be interpreted as providing a solution for situations where direct contact with outgroup members is not possible.

When the direct route is blocked: The extended contact pathway to improving intergroup relations

Interventions utilising contact with people with disabilities to improve children's attitudes towards disability: A systematic review and meta-analysis

As the number of people living with dementia increases, reducing stigma has become a policy priority. One way of decreasing stigma is through contact with the stigmatised group. However, the impact of this is difficult to establish due to a lack of validated measures suitable for adolescents. The aim of this study was to develop and validate a level of contact questionnaire designed to assess adolescents' contact with people living with dementia. Participants were recruited from five schools in two studies (N = 446 and N = 488) and completed the preliminary 11-item version of the adolescent level of contact of dementia (ALoCD). Study 1 explored the factor structure of the ALoCD, revealing two factors 'direct contact' and 'indirect contact'. Study 2 confirmed the structure of the ALoCD and tested for discriminant validity. These two studies resulted in a 9-item scale that showed adequate internal consistency (α = .89, α = .62) and discriminant validity between those who did and did not live with a person with dementia. The development of this scale enables assessment of direct (eg, living with a person with dementia) and indirect (watching a TV show about dementia) contact with dementia, and the extent of this contact. This initial validation suggests a psychometrically sound scale but further research should be undertaken to fully explore the properties of the scale.

Additive and multiplicative effects of different forms of positive and negative indirect intergroup contact in predicting intergroup attitudes

Research shows that direct and indirect intergroup contact reduces levels of prejudice towards immigrants. However, no research so far has explored the association of these different forms of contact with attitudes towards refugees. The present study analyses the relationship between the frequency and valence of direct intergroup contact with people with a migration background, the frequency of indirect contact with news on refugees, and the perception of realistic and symbolic threat, and attitudes towards refugees among adults in four European countries (Belgium, France, the Netherlands, Sweden). Data were collected in 2017 via online questionnaires ( N = 6,000). Using structural equation modelling, findings indicate that interethnic contact is positively related to attitudes towards refugees. Moreover, valence of direct contact is found to be more important to attitude formation than its frequency. Regarding indirect contact, exposure to news on refugees and public news consumption are positively related to attitudes, while commercial news consumption is negatively related to attitudes.

Sitophilus zeamais is a primary pest of maize. Our aim was to perform a qualitative review and meta-analyses with 56 scientific articles published from 1 January 2000 to 1 October 2022 dealing with direct (topical application) and indirect (impregnation of essential oils, EOs, onto filter paper or maize grains) contact toxicity of EOs against S. zeamais. Three independent meta-analyses of single means of LD50 (direct contact) and LC50 (indirect contact) were conducted using a random effect model. Essential oils more frequently evaluated were those belonging to Asteraceae, Apiaceae, Lamiaceae, Myrtaceae, Piperaceae, and Rutaceae. The LC50 global mean values were 33.19 µg/insect (CI95 29.81-36.95) for topical application; 0.40 µL/cm2 (CI95 0.25-0.65) for filter paper indirect contact; and 0.50 µL/g maize (CI95 0.27-0.90) for maize grains indirect contact. The species Carum carvi, Salvia umbratica, Ilicium difengpi, Periploca sepium, Cephalotaxus sinensis, Murraya exotica, Rhododendron anthopogonoides, Ruta graveolens, Eucalyptus viminalis, Ocotea odorifera, Eucalyptus globulus, Eucalyptus dunnii, Anethum graveolens, Ilicium verum, Cryptocarya alba, Azadirachta indica, Chenopodium ambrosioides, Cupressus semperivens, Schinus molle, Piper hispidinervum, Mentha longifolia, and Croton pulegiodorus showed LC50 or LD50 values lower than the global means, indicating good insecticidal properties. Our results showed that EOs have great potential to be used as bioinsecticides against S. zeamais.

To evaluate the role of child care centers in a community-wide hepatitis A epidemic. We analyzed surveillance data during an epidemic in Maricopa County, Arizona, from January to October 1997 and conducted a case-control study using a sample of cases reported from June to November. Cases were physician-diagnosed and laboratory confirmed; control subjects were frequency matched by age and neighborhood. Information regarding hepatitis A risk factors, including child care-related exposures, was collected. Characteristics of all licensed child care centers in the county were obtained through review of computerized lists from the Arizona Office of Child Day Care Licensing. Surveillance data were linked to the child care list to determine which centers had reported hepatitis A cases. We conducted univariate and multivariate conditional logistic analyses and calculated population attributable risks (PAR). In total, 1242 cases (50/100 000 population) were reported. The highest rates occurred among people aged 0 to 4 (76/100 000), 5 to 14 (95/100 000), and 15 to 29 (79/100 000) years. The most frequently reported risk factor was contact with a hepatitis A patient (45%). However, nearly 80% of these contacts were with individuals who attended or worked in a child care center. Overall, child care center-related contact could have been the source of infection for 34% of case-patients. In the case-control study, case-patients (n = 116) and control subjects (n = 116) did not differ with respect to demographic characteristics. A total of 51% of case-patients compared with 18% of control subjects reported attending or working in a child care setting (direct contact; adjusted odds ratio [OR]: 6.0; 95% confidence interval [CI]: 2.1-23.0) or being a household contact of such a person (indirect contact; OR: 3.0; 95% CI: 1.3-8.0). In age-stratified analyses, the association between hepatitis A and direct or indirect contact with child care settings was strongest for children <6 years old and adults aged 18 to 34 years. Household contact with a person with hepatitis A also was associated with hepatitis A (OR: 9.2; 95% CI: 2.6-58.2). The presence of a child <5 years old in the household was not associated with hepatitis A. The estimated PAR for direct child care contact was 23% (95% CI: 16-34), for indirect child care contact was 21% (95% CI: 13-35), and for any child care contact was 40% (95% CI: 30-53). Information on 1243 licensed child care centers was obtained, with capacity ranging from 5 to 479 slots (mean: 87). Thirty-four (2.7%) centers reported hepatitis A cases. Centers that had a mean capacity of >50 children were more than twice as likely to have had a reported case of hepatitis A (OR: 2.6; 95% CI: 1.1-6.7). Among the 747 centers that accepted >50 children, having infant (OR: 3.7; 95% CI: 1.6-8.3), toddler (OR: 6.3; 95% CI: 2.2-20.0), or full-day service (OR; undefined; 95% CI: 1.7- ~) was associated with having a reported case of hepatitis A. In Maricopa County, people associated with child care settings are at increased risk of hepatitis A, and child care attendees may be an appropriate target group for hepatitis A vaccination. Considering the estimated proportion of children who attended child care and were old enough to receive hepatitis A vaccine (>/=2 years of age) and the calculated PAR, approximately 40% of cases might have been prevented if child care center attendees and staff had been vaccinated. However, epidemiologic studies indicate that the proportion of cases that are attributable to child care center exposure varies considerably among counties, suggesting that this exposure may be associated with an increased risk of hepatitis A in some communities but not in others. To prevent and control hepatitis A epidemics in communities, the Advisory Committee on Immunization Practices and the American Academy of Pediatrics have adopted a long-term strategy of routine vaccination of children who live in areas with consistently elevated hepatitis A rates. After demonstrating cost-effectiveness, a rule was implemented in January 1999 to require hepatitis A vaccination of all children who are aged 2 to 5 years and enrolled in a licensed child care facility in Maricopa County. Other communities with similar epidemiologic features might consider routine vaccination of child care center attendees as a long-term hepatitis A prevention strategy. Consistent with current recommendations, in communities with persistently elevated hepatitis A rates where child care center attendance does not play an important role in hepatitis A virus transmission in the community, child care centers may nonetheless provide a convenient access point for delivering hepatitis A as well as other routine childhood vaccinations.