Evaluación del proceso fermentativo en la producción de hidromieles monoflorales colombianas

The quality of the raw material defines the industrial potential production. The harvest of raw sugarcane promoted a favorable environment for the increase of Mahanarva fimbriolata (Stål, 1854) pest that affects the plant development. This research was undertaken to evaluate the effects of M. fimbriolata on sugarcane quality and fermentation process. For the technological analysis, four damage levels and two evaluation periods were evaluated, while four fermentation cycles and two evaluation periods were tested for microbiological analysis, with three replications. The determined parameters were Brix (soluble solids), Pol (apparent sucrose), Purity, reducing sugars (RS), total reducing sugars (TRS), Total Acidity, pH and Total Phenolic Compounds in juice. In the fermentation process cellular viability analysis, yeast sprout and bacterial concentration were carried out. The wine was submitted to alcohol content, TRRS (total residual reducing sugars) and fermentation efficiency. The treatment with 60% of damage steams exhibited the lowest averages of Brix, Pol, Purity and TRS of the juice. An increase of the RS% of the juice and content of total phenolic compounds was observed. The cellular and sprouts viability were reduced with the increase in the damages caused by M. fimbriolata. The fermentation process exhibited larger quantities of contaminators. With the damages, the wine TRRS quantity was superior and, consequently there was a decrease of the alcoholic content and fermentation efficiency. The damages caused by M. fimbriolata affected the quality of the raw material, compromising the fermentative process, and increase the production of phenolic compounds and contaminators.

In order to screen high-quality processed tomato germplasm resources, the present research measured the content of quality indicators—lycopene, soluble solids, total acidity, total sugar, and vitamin C—in mature fruits of 113 processed tomato high-generation inbred lines. Comprehensive evaluations of germplasm quality were conducted through genetic diversity analysis, correlation analysis, principal component analysis, and cluster analysis. The results indicated that the variability of the five quality traits in the materials under test was relatively high, with a range of variation from 12.21% to 39.04%. Total sugar exhibited the greatest variation, while soluble solids content showed the least variation. The genetic diversity index ranged from 1.899 to 2.064, with total sugar, vitamin C, and lycopene showing high genetic variation. Soluble solids content was significantly positively correlated with lycopene, total sugar, and total acidity, while lycopene content was significantly positively correlated with total sugar. Vitamin C showed weaker correlations with other traits, but exhibited a significant negative correlation with total sugar. Total acidity had relatively simple correlations with other traits, being significantly correlated only with soluble solids. The three principal components extracted from the principal component analysis all had eigenvalues above 0.8%, contributing to a cumulative contribution rate of 77.435%. Through cluster analysis, the tested materials were divided into six major groups at an Euclidean distance of 15. Group I serves as candidate materials for breeding varieties with good basic quality and high vitamin C content. Group II stood out in terms of high sugar and lycopene content, suitable for developing tomato sauce or juice products with high vibrancy and sweetness. Group III had a high nutritional value and vibrant color, serving as core germplasm resources for breeding high-end processing-specific varieties. Group IV had high soluble solids content, making it a parent source for improving the viscosity and flavor of sauce tomatoes. Group V was suitable for specific formulations requiring high acidity or as breeding materials for high-acidity characteristics. Group VI had limited processing potential and should be used cautiously in breeding. The comprehensive evaluation results showed that the top five germplasm resources in terms of score were W119, 61, 82, 83, and W144. This study enriched the high-quality processed tomato germplasm resources and provided parental resources for quality breeding of processed tomatoes.

Mayo Clinic, Rochester, Minnesota. roy.tk@mayo.eduCapnography: Clinical Aspects. Edited by J. S. Gravenstein, M.D., Dr. med. h.c., Michael B. Jaffe, Ph.D., and David A. Paulus, M.D. Cambridge, United Kingdom, Cambridge University Press, 2004. Pages: 441. Price: $120.00.Practicing anesthesiologists and intensivists have come to take capnography for granted in the monitoring of surgical and critically ill patients. Although many standard anesthesiology texts contain a chapter about this important and useful technique, a comprehensive up-to-date treatment of the subject is not easy to find. Capnography: Clinical Aspects fills this void.The book is a multiauthored effort edited by two academicians and an engineer working in industry. The editors acknowledge significant overlap between chapters and characterize the book as more of a “symposium” than a textbook. There is adequate continuity of style between chapters, but as with any book written in this format, some chapters are more interesting to read than others.The book is organized into four parts. The first part is meant to be clinical and describes the interaction of respiratory, cardiovascular, and metabolic systems in determining the amount of exhaled carbon dioxide as measured by capnography. This is followed by parts on basic carbon dioxide physiology, the history of capnography, and the technology of capnography.The clinical part is divided into four sections: Ventilation, Circulation, Metabolism, and Organ Effects. The ventilation section is further divided into subsections on breathing assessment, airway management, monitoring of ventilation, weaning, and special situations. The first chapter (written by two of the editors) is a well-written introduction to time-based capnogram interpretation, the most commonly used form of capnography in the operating room setting. Of particular value is the introduction to the volume-based capnogram, a topic not commonly detailed in anesthesia texts. Subsequent chapters discuss capnography outside the operating room and in the prehospital setting for airway management, in particular to confirm tracheal intubation. The chapter on airway management in the intensive care unit includes a section on using capnography to confirm proper orogastric and nasogastric tube placement. The chapter on airway management in the operating room includes sections on confirming tracheal intubation and recognizing endobronchial tube placement.The chapter describing the use of capnography to monitor ventilation during anesthesia includes interesting comments on the Food and Drug Administration checkout relevant to capnography. This chapter also includes sections on equipment troubleshooting and how capnograms can be affected by positioning, pulmonary pathology, and several particular situations such as one-lung ventilation, laparoscopy, neurosurgery, cardiac surgery, tourniquet release, and high-frequency jet ventilation. Other chapters in this section focus on the use of capnography during transport and how it can be used in the field as a way to avoid deleterious effects of unintentional hyperventilation after intubation.A particularly comprehensive chapter describes the unique physiology and technological limitations of capnography in neonates and infants. Other chapters describe capnography in the sleep laboratory, capnography as a feedback tool for behavioral therapy in various disorders, and how the capnogram is affected by alterations in physiologic and technical limitations in high- and low-pressure environments.Chapters are also included on sedation and noninvasive ventilation. These chapters are valuable for their descriptions of how end-tidal carbon dioxide can be sampled during spontaneous ventilation in nonintubated patients and the clinical utility and limitations of end-tidal carbon dioxide as a method of estimating arterial carbon dioxide tension (PCO2) in noninvasive ventilation.Chapters relevant to critical care describe the use capnography to optimize tidal volume, alveolar minute ventilation, and positive end-expiratory pressure to wean patients from mechanical ventilation. These chapters also describe the use of volumetric capnography to assess carbon dioxide production and how the capnogram is affected by positive end-expiratory pressure, unilateral lung injury, tracheal gas insufflation, and various high-frequency ventilation modes.The circulation subsection includes chapters on how end-tidal carbon dioxide monitoring can be used to assess circulatory status during cardiopulmonary resuscitation and for prognostication during cardiac arrest in medical patients as well as the use of end-tidal and tissue carbon dioxide monitoring techniques to assess oxygen delivery in shock states. This section includes an elegant physiologic description of changes in alveolar dead space with pulmonary embolism and the use of capnography in diagnosis and treatment of pulmonary emboli and gas embolization in addition to a chapter on the utility of volumetric capnography for estimating arterial PCO2in patients with acute respiratory distress syndrome.The chapter on noninvasive pulmonary blood flow measurement describes complete and partial carbon dioxide rebreathing techniques as alternatives to invasive cardiac output monitoring. A variety of clinical scenarios illustrating the use of these techniques sets this chapter apart from other descriptions of this topic.The metabolism subsection includes a single chapter describing alterations in normal physiology induced by surgery and anesthesia that affect carbon dioxide elimination. The chapter discusses alterations in ventilation, circulation, and carbon dioxide metabolism that are influenced by temperature alterations, various anesthetic techniques, and pharmacologic agents as well as particular intraoperative situations such as laparoscopy, tourniquet release, vascular cross clamping, and cardiopulmonary bypass.The final chapter of the ventilation section describes the effects of hypercapnia and hypocapnia on tissue oxygenation and perfusion, focusing on the central nervous system, respiratory system, and cardiovascular system. This is an excellent introduction to the effect of carbon dioxide at the organ, tissue, and cellular/molecular level and could have been included in the section on physiology.The physiology section includes a chapter on carbon dioxide pathophysiology, which describes inherited and acquired mitochondrial and enzyme disorders as well as pharmacologic agents that alter carbon dioxide production. The chapter also discusses carbon dioxide embolism and the increase in PCO2during apnea testing for brain death. There is a complete if somewhat standard chapter on acid–base physiology, followed by an excellent description of how capnography can provide information on ventilation/perfusion mismatch from a physiologic standpoint, including examples of various disease states. Subsequent chapters describe clinical correlates of alterations in normal time and volume capnographic tracings and how capnograms can provide clues to the underlying pathophysiology.A particularly interesting chapter in this section summarizes a biomedical engineering approach to illustrate the underlying anatomical and physiologic processes that result in a normal volumetric capnogram. A mathematical model that accounts for bronchial airway structure, gas convection and diffusion, and the carbon dioxide release from alveolar capillary blood is shown to generate a computed washout curve that shows remarkable agreement with an experimentally measured capnogram from a healthy human subject. This illustrates the utility of physiologic modeling as a useful tool for investigating potentially complex pathophysiologies without placing patients at risk.A unique historical section describes the evolution of time and volumetric capnography with many interesting anecdotes, as well as a first-person account by Smalhout, an early proponent of capnography. A selection of capnographic tracings corresponding to clinical events that he made over a 20-yr period is one of the highlights of this book. Without reading this section of the book, few people would realize that the impetus for carbon dioxide analyzer development was to investigate the cause of death in patients who turned out to be rebreathing due to a channeling issue through carbon dioxide absorption devices, or that carbon dioxide analyzers enabled a reduction in mortality for polio patients by allowing clinicians to titrate ventilation to expired carbon dioxide instead of adjusting ventilation based on their weight.The technological section fulfills the editors’ wishes for providing clinicians with information necessary to appreciate the mechanism, design, and limitations of devices for measuring carbon dioxide. Various chapters address technical specifications and standards (e.g. , accuracy, range, drift, response time, interfering gases, alarm systems, calibration) for carbon dioxide analyzers and describe technological limitations for flow measurement, required to estimate carbon dioxide production. Another chapter describes various methods for carbon dioxide detection, including infrared, photoacoustic, colorimetric, and mass spectrometry methods. Unfortunately, Raman spectroscopy is not included simply because it is not currently commercially available. This chapter also includes a discussion of mainstream versus sidestream carbon dioxide analyzers.The book ends with a mini-atlas of capnographic waveforms typifying various physiologic states, which is useful although not exhaustive.As the editors acknowledge, there is a fair amount of redundancy; as an example, the fact that highly sensitive colorimetric carbon dioxide indicators can yield false positives with esophageal intubation is mentioned in multiple chapters along with the fact that false negatives in cardiac arrest have led to the removal of correctly placed endotracheal tubes. Other recurring themes include the predictive value of end-tidal carbon dioxide in assessing arterial PCO2and the utility of volumetric capnography. In general, I found the multiple perspectives to be helpful instead of confusing or irritating. As with any book, the onus is on the reader to formulate his or her judgment with the assistance of the most recent literature.The overall introduction to the book and the introduction chapters for each section are very short and could have been used to provide the reader with a more substantial description of the basic concepts or objectives of each section. The section and subsection titles are somewhat arbitrary, and some chapters are in fact assigned to their own sections. Although the terminology is relatively consistent, the book could also use a more comprehensive list of abbreviations and acronyms used in various chapters. I found most of the typographical and page-setting errors to be minor (with the exception of a reference to “title” volumes). In spite of these limitations, the book admirably maintains its focus on capnography; readers interested in the latest tissue oxygen tension (PO2) monitoring techniques, for example, will have to look elsewhere.In summary, Capnography: Clinical Aspects is a very readable introduction to a topic addressed by few textbooks. It is useful as a reference primarily because of its comprehensive index and contains much information useful to the practitioner of critical care as well as anesthesiology. It addresses the physiologic and technological considerations that need to be understood to make capnography a clinically useful tool and should be standard reading for those who depend on it as a basic anesthetic monitor.Mayo Clinic, Rochester, Minnesota. roy.tk@mayo.edu

Fermented cereal-based drinks as “kounou” are among the most popular indigenous beverages in Northern Cameroon. Because they are made in a primitive way, they are ready to be served while still actively fermenting. Therefore, some physicochemical properties of “kounou” were tested in the current study during 4 days of storage at room and refrigerated temperatures. Samples of “kondourkou” a sub type of “kounou” were purchased from local producers located in the Far North region of Cameroon. During storage at 28 ± 2°C and 4 ± 1°C, total sugars, pH, total acidity, alcohol content, total soluble solids, dry matter, total dissolved solids, and conductivity of samples were measured in triplicate using standards procedures. The results showed that pH decreased from 3.34 to 3.16, and 3.65 to 3.4 after storage at room and refrigerated temperatures, respectively. The decrease in total sugar (30.3%-12.1%) and soluble solids (7.26-4.89°Brix), as well as increase in titratable acidity (0.26%-0.36%) were significant for samples stored at ambient temperature. While no significant changes in all these parameters were revealed for samples stored at cold conditions (54.8%-37.9%; 8.79-8.13°Brix and 0.18-0.25%, respectively). Dry matter and total dissolved solids were lower in samples stored at room temperature (from 10.3-11.2% and 841.9-1002 mg/L, respectively) than those kept under refrigeration (from 12.15 to 12.5% and 1061.2-1231.7 mg/L, respectively). Alcohol content and conductivity were respectively in the range of 3.5-2.35% and 1692.4-2012.5 mg/L for samples stored at room temperature, and 3.18-3.37% and 1692.4-2205 mg/L for those kept under refrigeration. Principal component analysis revealed that samples stored under refrigeration were structured in two sub-cluster, the first formed by those stored for 1 and 2 days and the second including those kept for 3 and 4 days. While samples left at room temperature were separated into three sub groups, those stored for a day, 2 and 3 days, and 4 days. Finally, it would be appropriate to consume refrigerated “kounou” as well as commercialize and/or store it under refrigeration.

Simultaneously calibrating solids, sugars and acidity of tomato products using PLS2 and NIR spectroscopy

The umbu is the umbuzeiro fruit ( Spondias tuberosa Arr. Cam.) which is a tree native of the Brazilian backlands. The aim of this work was to evaluate the physical and chemical properties of commercial and concentrated umbu pulp to be used as raw material in the production of structured. The commercial pulp (2.5 kg) was acquired in the trade of Feira de Santana. The concentration of the evaporator route pulp vacuo to increase the soluble solids content of 8.5oBrix to 15.5oBrix It was held in the Food Chemistry Laboratory of the State University of Feira de Santana. It was obtained after concentrating a yield of 41.12% by mass of fruit pulp. The concentration process had no influence in the nutritional value of umbu pulp. The concentrated pulp had the following properties pH (2.43), soluble solids (15.5°Brix), acidity (3.11 g citric acid/100 g), total protein (1.28% - w/v), vitamin C (4.36 mg/100 g - w/v), reducing sugars (6.08% - w/v), total sugars (13.55% - w/v) non reducing sugars (7.46% - w/v) and ash (0.42% - w/v). The physicochemical characteristics of commercial umbu pulps had similar pH, Total Soluble Solids, acidity, proteins and ashes; higher values of reducing sugars and non-reducing and a lower vitamin C content that the pulps reported in the literature. The concentration process produced an increase in Total Soluble Solids, proteins, acidity, reducing and non reducing sugars and ash content. However there was a decrease in vitamin C concentration of the pulp.

The jaboticaba is a fruit tree with great potential, both for fresh consumption, as for industrialization. The objective of this study was to evaluate the shelf-life of jaboticabas’s fruits cv. ‘Sabará’ stored under refrigeration and at room temperature. After harvest, the fruits were washed, screened, packed in plastic trays, in single layer, and stored under refrigeration (5 °C, and 49% UR), and at room temperature (27 ºC, and 33% UR). The determinations performed in triplicate immediately after harvest and every other day, until the disposal of the fruit, were: pH, soluble solids (°Brix), total titratable acidity (% citric acid), total and reducer sugars (%), vitamin C (mg/100g) and phytopathological. The design adopted was entirely randomized, realized the analysis of variance (F test) and comparison of averages by Tukey test to 5% of significance. Under refrigeration, the storage was more than 9 days to 12 days, the fruits showed intense shriveling (loss of moisture), being unsuitable for marketing. The soluble solids remained stable during this period, but there was decrease of pH and the levels of total and reducer sugars, in addition to increased acidity, facts that indicate occurrence of fermentation process. The losses of vitamin C were of the order of 72,5, from 4,952mg/100g in zero time, and 3,593mg/100g at the end of this period. The stored fruits at room temperature were discarded at the beginning of storage, by shriveling, incidence of microorganisms and strange odor.

Sucrose, fructose, total sugars and soluble solids are major factors in determining mature melon fruit sweetness. Bulked segregant analysis was utilized to detect RAPD markers associated with QTL for sucrose, total sugars and soluble solids in an F2 population from the ananas melon cross of Deltex (high sugars) × TGR1551 (low sugars). Sucrose, glucose, fructose and total sugar data were obtained from 108 F2 plants by means of HPLC. Clear separations for sucrose, total sugars and soluble solids between Deltex and TGR1551 were observed, whereas slight differences for glucose and fructose were found. Continuous distributions for sucrose, total sugars and soluble solids were observed in the F2 population indicating quantitative inheritance for the sweetness traits. A significant negative correlation was observed between sucrose and glucose (r = -25) or fructose (r = -0.31). A significant positive correlation was noted between sucrose and total sugars (r = 0.80) or soluble solids (r = 0.64). Three low and high DNA bulk pairs for sucrose, total sugars and soluble solids were developed. A total of 360 primers were used to simultaneously screen between the low and high bulks, and between Deltex and TGR1551. Sixty-eight RAPD markers were polymorphic for the low and high bulks. Of the 68 markers, 24 were found to be significantly associated with sucrose, total sugars or soluble solids on the basis of single-factor ANOVA. Marker OM15.550 was consistently associated with QTL affecting sucrose, glucose, fructose, total sugars and soluble solids, and accounted for 7% to 25% of the phenotypic variation for the traits. These markers associated with the sugar synthesis QTL could be useful to transfer these genes into a low sugar cultivar to enhance the fruit sweetness.

The physiology and biochemistry of muskmelon (Cucumis melo L.) fruit were studied in combination with nitrogen and calcium nutrition. Two forms of nitrogen ((NH4)2SO4 and NH4NO3) and 2 sources of calcium (CaCO3 and CaCl2) were studied with particular reference to carbon dioxide and ethylene production. In addition, fruit ethanol, chlorine and sugar contents were also estimated. Calcium, when applied in the form of CaCl2, was detrimental, in that, it produced more carbon dioxide and ethylene, decreased the mean fruit weight and also advanced the respiration peak by about 2 days to the ninth day after harvest compared with the 11th day in CaCO3. In this case, very high fruit ethanol and chlorine content seemed to affect the fruit quality. Such detrimental results were not observed in CaCO3 treatments. Irrespective of the treatments, carbon dioxide and ethylene production curves followed a typical sigmoidal pattern, confirming the climacteric nature of the fruit. Carbon dioxide and ethylene production, total soluble solid and ethanol contents, and ethanol and chlorine contents were positively correlated. Carbon dioxide production and fruit weight, and fruit weight and chlorine content were negatively correlated.

Background: Vinegar could be counted as one of the oldest acetic acid diluted solution products among the history. It can be produced by various fermentation methods using any sugary substrate, especially fermented fruit juice. Due to the presence of many kinds of compounds which formed in the fermentation process, the final vinegar products could show unique functions. The aim of current study was to produce a new type of vinegar kombucha using different fruit juices substrates. Methods: Kombucha vinegar was obtained by fermentation of kombucha broth layer on sweetened pasteurized fruit juices (pomegranate, red grape, sour cherry and apple). Fermentation took place in 4 L glass vessel and fortified with 2% of previously processed broth. Incubation was carried out at 27±2 °C for 14 days. Several factors such as; pH, total acidity, and acetic acid content, invert sugars, remaining sucrose, alcohol, soluble solids and the yield of biomass were measured periodically (0, 3, 7, 10 and 14 days after processing). Results: As the fermentation continues, the actual concentration of acids, pH value, fructose content and biomass yield significantly increased (P<0.01) but the alcohol and residual sucrose content decreased in all the juices. Contents of acetic acid on 14th day of fermentation were 3.8, 3.6, 3.5 and 3.4 g/100 ml of pomegranate, red grape, sour cherry and apple juice vinegar, respectively. Moreover, soluble solid in kombucha vinegar was determined (24.7, 38.1, 19.1 and 36.5 g/100 ml in pomegranate, red grape, sour cherry, and apple, respectively). Conclusion: The composition of different kombucha preparations is greatly affected by the individual kombucha layer used. Keywords: Acetic acid, beverages, fermentation, fruit juice, kombucha vinegar, substrate.

Quality control of cashew apple and guava nectar by near infrared spectroscopy

Pepper can be expanded into processed products such as food and beverages. Kombucha is one of the products that can be made with pepper basic ingredients. Fermented kombucha tea has been shown to boost antioxidant activity. The presence of free phenolic substances obtained during the fermentation process causes an increase in antioxidant activity. The purpose of this study was to determine how pepper shape (powder and granule), concentration (5 and 10 g), and formulation (pepper alone and pepper coupled with black tea) affected the physicochemical properties of pepper-based kombucha. Kombucha was made by fermenting a 20% sucrose solution with a bacterial and yeast symbiotic culture (SCOBY) for 14 days. The parameters tested were pH, total sugar (°Brix), and total titratable acidity. The result of this study is the addition of 10 grams of pepper granules resulted in the lowest pH value (3.43), the lowest total sugar was in the treatment of tea pepper powder adding 10 grams (15.00) and the highest was a mixture of tea pepper powder 5 grams (17.87 Brix). The third anlaysis is total acid showed the lowest total acid value in the tea pepper granule treatment with the addition of 5 grams (70.74 %) and the highest, namely a mixture of tea. pepper granules 10 grams (94.61 %). Based on overall physicochemical characteristics, the addition of 10 g pepper granules produced the best kombucha quality. These findings indicate that white pepper has strong potential as a raw material for kombucha fermentation

Anaerobic Metabolism During Cardiopulmonary Bypass: Predictive Value of Carbon Dioxide Derived Parameters

Forest soil profiles of two dunes within the European belt of inland dunes were analysed in the laboratory. We carried out respirometric measurements of carbon dioxide production and oxygen consumption for every horizon of the studied soils while simultaneously quantifying the organic matter and humidity. Oxygen consumption and carbon dioxide excretion decreased exponentially with depth. The oxygen consumption decrease was less rapid than the decrease in carbon dioxide production. We found a statistical significant linear dependence between oxygen consumption and carbon dioxide excretion, and organic matter content and soil water capacity. Respiration processes in the profiles were divided into two strata; oxygen respiration dominated in the first and fermentation processes in the second. We estimated total respiration in the studied profiles for an area of 1 m2 down to around 1 m depth. We concluded that when assessing the soil’s role in carbon cycling in an ecosystem, it is necessary to consider both the respiratory and fermentation strata, as both produce large quantities of carbon dioxide. The main factor determining carbon dioxide production intensity is organic matter content; thus the distribution of organic matter in the soil profile determines carbon cycling intensity.

In order to predict storage life of navel orange, The model for the variable regularity of total soluble sugar, total acidity, vitamin C, soluble solids, the sugar-acidity ratio in navel orange according to storage time was established based on BP artificial neural network . The results show that the multi-factor BP artificial neural network model has better predicted effect than single-factor one. When the number of the hidden layer neuron is 8, the multi-factor BP artificial neural network model of total soluble sugar, total acidity, vitamin C, soluble solids, the sugar-acidity ratio according to storage time was the most accurate, the correlation coefficient R between prediction and true value of storage time reached 0.98, the prediction and true value of the model was 0.99. As a result, the multi -factor BP artificial neural network model could be used to predict the navel orange storage life.