Abstract
Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of Mandevilla moricandiana ethyl acetate fraction and subfractions. Vascular effects were investigated on aorta isolated from control and monosodium glutamate (MSG) induced-obese Wistar rats, and antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods. The ethyl acetate fraction (MMEAF) induced a concentration-dependent vasodilation on aortic rings through the NO pathway, with the involvement of histamine H1 and estrogen ERα receptors and showed potent antioxidant activity. In aorta of MSG obese rats, maximal relaxation to acetylcholine was increased in the presence of MMEAF (3 µg/mL), indicating that MMEAF ameliorated obesity-induced endothelial dysfunction. Quercetin and kaempferol aglycones and their correspondent glycosides, as well as caffeoylquinic acid derivatives, A-type procyanidin trimer, ursolic and oleanolic triterpenoid acids were identified in subfractions from MMEAF and seem to be the metabolites responsible for the vascular and antioxidant activities of this fraction.
Highlights
Pretreatment with fulvestrant did not alter maximal vasodilator response but induced a rightward displacement of the concentration–response curve of MMEAF, increasing EC50 to 13.94 ± 3.44 μg/mL (p < 0.05; Figure 5B). These results indicate that the vasodilation induced by MMEAF is mediated, at least in part, through the activation of endothelial histamine H1 and estrogen receptors
The substitution of the hydroxyl group in C3 by sugars can reduces the antioxidant activity of flavonoids [72]. These data are in agreement with the results presented by the MMEAF-B fraction, which contains isoquercitrin and astragalin as major compounds, totaling 65.27% percentage of relative area, in addition to other glycosides, and presented lower antioxidant activity by the oxygen radical absorbance capacity (ORAC) method
We investigated the phytochemical and pharmacological properties of MMEAF in order to evaluate its potential as a source of phenolic compounds with vascular protective actions
Summary
Cardiovascular diseases (CVD) are the leading cause of death worldwide, being responsible for 17.9 million deaths in 2019 [1]. Endothelium dysfunction is involved in both the development and progression of CVD and is present in risk factors for these diseases. It plays a key role in structural and functional vascular changes observed in atherosclerosis [2,3], coronary artery disease [4], hypertension [5,6], and obesity [7,8]. The increasing incidence of obesity represents a global health problem since it is commonly
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
