Ethoxyzolamide Inhibition of CO2 Uptake in the Cyanobacterium Synechococcus PCC7942 without Apparent Inhibition of Internal Carbonic Anhydrase Activity

Abstract

In high inorganic carbon grown (1% CO(2) [volume/volume]) cells of the cyanobacterium Synechococcus PCC7942, the carbonic anhydrase (CA) inhibitor, ethoxyzolamide (EZ), was found to inhibit the rate of CO(2) uptake and to reduce the final internal inorganic carbon (C(i)) pool size reached. The relationship between CO(2) fixation rate and internal C(i) concentration in high C(i) grown cells was little affected by EZ. This suggests that in intact cells internal CA activity was unaffected by EZ. High C(i) grown cells readily took up CO(2) but had little or no capacity for HCO(3) (-) uptake. These cells appear to possess a CO(2) utilizing C(i) pump that has a CA-like function associated with the transport step such that HCO(3) (-) is the species delivered to the cell interior. This CA-like step may be the site of inhibition by EZ. Low C(i) grown cells possess both CO(2) uptake and HCO(3) (-) uptake activities and EZ inhibited both activities to a similar degree, suggesting that a common step in CO(2) and HCO(3) (-) uptake (such as the C(i) pump) may have been affected. The inhibitor had no apparent effect on internal CO(2)/HCO(3) (-) equilibria (internal CA function) in low C(i) grown cells.