Ethnobotanical Exploration and Formulation Development of Cream Containing Ammi majus Linn and Portulaca oleracea Linn

Abstract

Stearic acid served as the emulsifier in the preparation of an O/W emulsion-based cream that also included oil-soluble components and water-based extracts of Ammi majus Linn and Portulaca oleracea Linn. The cream was evaluated for color, appearance, presence of foreign particles/grittiness, pH, viscosity, phase separation, and extrudability. A skin irritation study was conducted on Wistar rats, with intact skin and hair removed three days earlier experiment. Cream-containing extracts were applied to test animals, and the backs of animals were scrutinized for erythema and edema. To study psoriatic skin cells in-vitro, researchers used the HaCaT cell line. Researchers also used the SRB assay to look for antipsoriatic effects in-vitro. Cultures of human keratinocyte cell lines HaCaT were carried out in a medium that included 10% fetal bovine serum and was otherwise identical to Dulbecco’s modified Eagle’s medium. In order to reach a concentration of 10%, 25 μL of 50% TCA was added to each well after 72 hours, creating a thin layer of ended drug dilutions. Phytochemical evaluation revealed that the extracts of A. majus Linn and P. oleracea Linn were soluble in water and had a yellowish solid color. The cream formulation showed good emulsionability, no foreign particles or grittiness, and no phase separation. The skin irritation test on Swiss albino rats showed that the standard drug (Retino A 0.05% cream) was free from irritation. The cream’s acceptability for topical use was also observed.