Abstract
Summary Adaptation of a double isotope ratio method to the reticulocyte cell-free translation assay has revealed two estrogen-regulated mRNA's in the human breast cancer cell line MCF-7. These mRNA's code for proteins of 24,000 and 54,000 daltons as analyzed on SDS polyacrylamide gels. The 24K and 54K message levels increase 1.9 and 1.7 fold, respectively, following 6 days of estrogen treatment in cells whose growth has been inhibited by antiestrogen pretreatment. These results extend our previous observation that estradiol rescue of antiestrogen-blocked cells stimulates the synthesis of a 24,000 dalton protein in MCF-7 cytosols. Evidence for transcriptional control of this protein makes it an attractive probe for the study of hormone-regulated gene expression in human breast cancer.
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