Abstract
We examined the effect of estrogen (E2) replacement in ovariectomized rats on food intake and lateral hypothalamic neuron′s activity during glucoprivation induced by i.v. 2‐deoxy‐D‐glucose (2DG) administration. Rats were ovariectomized and implanted E2 or vehicle (cholesterol) subcutaneously. Two weeks after the replacement, rats were infused with 2DG (400 mg/kg) and food intake was measured for 3 hours. The same experiment was performed for immunohistochemical examination of c‐Fos, melanin concentrating hormone (MCH), and orexin A expression in the lateral hypothalamic area (LH) and arcuate nucleus (Arc). Glucoprivation induced c‐Fos expression in the orexin A neurons locating at the perifornical region of LH, but did not activate MCH neurons. Glucoprivation significantly induced food intake, and food intake in E2 replaced group was significantly lower than E2 deficit group. The number and fraction of c‐Fos expressed orexinergic neurons was significantly lower in E2 replaced group than E2 deficit group. The number of c‐Fos ir cells was less in E2 replaced group than E2 deficit rats. These data indicate that E2‐induced reduction of food intake induced by 2DG is possibly due to the reduced perifornical orexinergic neuron′s activity and to the reduced Arc neuron′s activity.
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