Abstract
Introduction: This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning.
 Objectives: The purpose of the study was to evaluate the diagnostic capabilities of the azimuthal-invariant Muller-matrix polarimetry technique of the polycrystalline constituent of BT and human body fluids in determining the TSD in cases of alcohol and carbon monoxide poisoning.
 Results: A comprehensive experimental study of the diagnostic efficiency of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the brain, myocardium, adrenal glands, liver, and polycrystalline blood films from dead persons for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.A sensitivity range of 24 hours was determined using the method of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the myocardium for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.
 Conclusion: The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.
Highlights
This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning
Determining the TSD of a person continues to be one of the most important issues to be resolved during the forensic examination of a dead body
Attention is drawn to the lack of information on specific morphological changes in BT in various pathological conditions, such as, poisoning with alcohol and carbon monoxide (Hayden, 2019; Kim et al, 2018; Rose et al, 2017)
Summary
This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning. It is necessary to develop and implement new methods for determining the time since death. A new step was the application and development of laser polarimetric methods of microscopic examination of optically inhomogeneous biological structures using statistical analysis of digital polarization maps. The study of Ushenko (2015) confirmed that these methods provide almost complete information about the polycrystalline structure of BT and fluids of the human body and allow to examine the dynamics of changes in different pathological conditions for the diagnosis of the post-mortem interval
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