Abstract

In this paper, an approach to the estimation of multiple biomass growth rates and biomass concentration is proposed for a class of aerobic bioprocesses characterized by on-line measurements of dissolved oxygen and carbon dioxide concentrations, as well as off-line measurements of biomass concentration. The approach is based on adaptive observer theory and includes two steps. In the first step, an adaptive estimator of two out of three biomass growth rates is designed. In the second step, the third biomass growth rate and the biomass concentration are estimated, using two different adaptive estimators. One of them is based on on-line measurements of dissolved oxygen concentration and off-line measurement of biomass concentrations, while the other needs only on-line measurements of the carbon dioxide concentration. Simulations demonstrated good performance of the proposed estimators under continuous and batch-fed conditions.

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