Abstract
A phenotypic and molecular diversity study was conducted using seven traits and 19 SSR markers in a collection of 26 black gram genotypes. Phenotypic characterization was based on seven yield and yield related variable. The field experiment was laid out at Panboli village (YMV hotspot) of Tirunelveli District in Tamilnadu during summer 2017. Genetic divergence was estimated on the basis of D2 values and 26 genotypes under study were grouped into six clusters by Tocher’s method. Seed yield per plant followed by Plant height and number of pods per plant contributed to the genetic divergence. The genetic distance announced using DICE dissimilarity co-efficient indicated highest divergence of 1.0 between VBN 8 and AUBG 17 and between VBN 8 and AUBG 19. The dendogram constructed using the DICE dissimilarity co-efficient between genotypes showed four apparent clusters based on marker allele distribution. Divergence was noted between the dissimilarity matrices based on the molecular and phenotypic diversity based on agronomic data.
Highlights
Pulses are one among the important crops in the world
The first cluster consisted of six genotypes (AUBG 2, AUBG 7, AUBG 9, AUBG 12, AUBG 13 and AUBG 16) and accommodated 23% of the total population based on allelic similarity
The second cluster consisted of four genotypes having a membership density of 15.4%. It consisted of AUBG 3, AUBG 4, AUBG 10 and AUBG 11
Summary
Pulses are one among the important crops in the world. They are grown on 21-22 million hectares of area globally with an annual production of 12-16 million tonnes [1]. An attempt was made to assess the phenotypic diversity using morphological traits and to estimate genetic diversity of blackgram accessions using molecular markers. The dendogram constructed using the DICE dissimilarity co-efficient between genotypes showed four apparent clusters based on marker allele distribution. The first cluster consisted of six genotypes (AUBG 2, AUBG 7, AUBG 9, AUBG 12, AUBG 13 and AUBG 16) and accommodated 23% of the total population based on allelic similarity.
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