Abstract

Some physical properties of glycogen have been investigated and applied to micro isolation and assay in biological material containing only a few micrograms of glycogen. Losses of glycogen in the ethanol precipitation step were found to be preventable by the use of a coprecipitant, of which Na 2SO 4 was the most satisfactory. Evidence for the existence or formation of at least two water-insoluble glycogen complexes in liver was presented. Treatment at 100°C with concentrated KOH for 20 hr rendered all glycogen soluble in water.

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