Estimation of Crystallite Size, Lattice Strain and Dislocation Density of Nanocrystalline Carbonate Substituted Hydroxyapatite by X-ray Peak Variance Analysis

Abstract

X-ray peakprofile analysis by variance method is employed to evaluate the micro structural parameters namely crystallite size, mean squared lattice strain, root mean squared lattice strain and dislocation density of hydroxyapatite (HA) and carbonate substituted [3 wt% (3CHA) and 6 wt% (6CHA)] hydroxyapatite nanoparticles prepared by microwave synthesis technique. The calculated average crystallite sizes of HA, 3CHA and 6CHA by variance method are correlated with the values obtained from Scherer's and transmission electron microscopy (TEM) results. It is found that the results of the average crystallite size measured by variance analysis method are in good agreement with TEM results. The calculated micro structural parameters are also correlated with the in-vitro dissolution study results. It is found that variance method of X-ray peak profile analysis is more appropriate to quantify the micro structural parameters of nanostructured hydroxyapatite based nanoparticles.