Abstract
The function of the human body are controlled by enzymes and hormones. Similarly, in the laboratory, the enzyme, glucose oxidase, is used to hydrolyse glucose. In the human body, glucose and insulin control blood glucose levels, which are dependent on food intake. In this study, the enzyme specificity and glucose concentration of sugar solutions would be estimated. In order to achieve this, an experiment involving the use of indicator strips and a two-fold serial dilution was performed. Furthermore, conclusions on blood glucose and serum insulin levels would also be discussed from a given data set.
Highlights
Enzyme specificity refers to the selectivity of an enzyme to a specific substrate
Enzymes act on substrate in wither a lock-and-key theory of enzyme action or through chemical modification (Becker et al, 2003)
Enzyme specificity estimation Four strips of filter paper were labelled to the sugar solution to be tested
Summary
Enzyme specificity refers to the selectivity of an enzyme to a specific substrate. The substrate can be anything, usually a substrate or substance, on which the enzyme is able to initiate a chemical reaction (Singh, 2019). In order to estimate the enzyme specificity and glucose concentration of major solutions, a glucose detection system was made. The products react with oxygen and water to produce a colour change, and the glucose concentration of a sugar solutions can be estimated quantitatively by assessing the intensity of the colour change. Enzyme specificity estimation Four strips of filter paper were labelled to the sugar solution to be tested.
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